ICC/IF image mouse anti GFAP monoclonal antibody 5C10 3743

IHC image mouse anti GFAP monoclonal antibody 5C10 3743

mouse anti-GFAP monoclonal antibody (5C10) 3743

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to GFAP
Suitable for: WB, ICC/IF, IHC
Reacts with: human, mouse, rat
Isotype: IgG1
100 µg, 25 µg, 1 mg

available sizes

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SKU: 3743parent Categories: antibody, cell marker Tag: GFAP

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

mouse anti-GFAP monoclonal antibody (5C10) 3743

antibody
Database link: human P14136 mouse P03995 rat P47819
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:5000 IF/ICC or IHC: 1:1000
Immunogen Purified porcine spinal cord GFAP
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
Glial Fibrillary Acidic Protein (GFAP) is a crucial cell marker widely employed in neuroscience and neurobiology research. It is an intermediate filament protein primarily found in astrocytes, a type of glial cell in the central nervous system. GFAP serves as a reliable marker for identifying and characterizing astrocytes in various experimental models and tissues. Immunohistochemistry and immunofluorescence techniques using antibodies targeting GFAP enable researchers to visualize and quantify astrocytic populations, gaining insights into their distribution, morphology, and function in the brain. Moreover, GFAP antibodies are instrumental in studying astrocyte reactivity, a phenomenon occurring in response to brain injury or neurodegenerative diseases. As astrocytes play critical roles in neural development, neurotransmitter regulation, and maintenance of the blood-brain barrier, GFAP has proven indispensable in advancing our understanding of glial biology and its contribution to neurological disorders. Click for more on: cell markers and GFAP
Protein names Glial fibrillary acidic protein (GFAP)
Gene names GFAP,GFAP
Protein family Intermediate filament family
Mass 49880Da
Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subellular location Cytoplasm Note=Associated with intermediate filaments.
Tissues Expressed in cells lacking fibronectin.
Structure Interacts with SYNM. ; [Isoform 2]: Interacts with PSEN1 (via N-terminus).
Post-translational modification Phosphorylated by PKN1.
Involvement in disease Alexander disease (ALXDRD) [MIM:203450]: A rare disorder of the central nervous system. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death within the first decade. Infants with Alexander disease develop a leukodystrophy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. Histologically, Alexander disease is characterized by Rosenthal fibers, homogeneous eosinophilic inclusions in astrocytes. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P14136
The UniProt Consortium

Data

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to GFAP, 3743, dilution 1:1,000 in red, and costained with rabbit pAb to NF-L, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. The 3743 antibody stains astrocytes, while antibody reveals dendrites and axons of neurons.

This is one of a series of formalin fixed paraffin embedded adult horse brain samples generated by Maureen T. Long in the Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida. The findings were recently published in the Equine Veterinary Journal, link here. The brains from this series were removed whole, sliced incompletely cross ways and fixed over a 10-20 day period. The whole brain was put in a large necropsy specimen bucket containing 1-2 gallons of fixative, and after 24 hours, the formalin was poured off and then fresh formalin was added. The samples were fixed and embedded in paraffin in 2007 and sections were kept at room temperature until 2011. The sections were processed for antigen retrieval by boiling in pH=6 Citrate buffer for 10 min. Primary incubation with 3743 was for 1 hour at 37�C, and secondary antibody incubation and color reaction was performed using the Vector mouse 8718C kit.

Paraffin embedded formalin fixed human cerebellum stained with 3743. The sample was processed using our standard IHC protocol outlined here. The folds of the molecular layer are at the right and granular and white matter layers are on the left. Section was lightly counterstained with hemotoxylin.

Western blot analysis of whole tissue lysates using mouse mAb to GFAP, 3743, dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 50kDa corresponds to the GFAP protein.

Publications

pmid	title	authors	citation
37237366	Reactive Bergmann glia play a central role in spinocerebellar ataxia inflammation via the JNK pathway.	Chandrakanth Reddy Edamakanti, Vishwa Mohan, Puneet Opal	J Neuroinflammation 20:126
35839339	Enhanced nociceptive behavior and expansion of associated primary afferents in a rabbit model of cerebral palsy.	Emily J Reedich, Landon T Genry, Meredith A Singer, Clarissa Fantin Cavarsan, Elvia Mena Avila, Daphne M Boudreau, Michael C Brennan, Alyssa M Garrett, Lisa Dowaliby, Megan R Detloff, Katharina A Quinlan	J Neurosci Res 100:1951-1966
35203460	Mitoquinone Helps Combat the Neurological, Cognitive, and Molecular Consequences of Open Head Traumatic Brain Injury at Chronic Time Point.	Muhammad Ali Haidar, Zaynab Shakkour, Chloe Barsa, Maha Tabet, Sarin Mekhjian, Hala Darwish, Mona Goli, Deborah Shear, Jignesh D Pandya, Yehia Mechref, Riyad El Khoury, Kevin Wang, Firas Kobeissy	Biomedicines 10:N/A
35065054	Mitoquinone supplementation alleviates oxidative stress and pathologic outcomes following repetitive mild traumatic brain injury at a chronic time point.	Maha Tabet, Marya El-Kurdi, Muhammad Ali Haidar, Leila Nasrallah, Mohammad Amine Reslan, Deborah Shear, Jignesh D Pandya, Ahmed F El-Yazbi, Mirna Sabra, Stefania Mondello, Yehia Mechref, Abdullah Shaito, Kevin K Wang, Riyad El-Khoury, Firas Kobeissy	Exp Neurol 351:113987
33124191	SATB2 drives glioblastoma growth by recruiting CBP to promote FOXM1 expression in glioma stem cells.	Weiwei Tao, Aili Zhang, Kui Zhai, Zhi Huang, Haidong Huang, Wenchao Zhou, Qian Huang, Xiaoguang Fang, Briana C Prager, Xiuxing Wang, Qiulian Wu, Andrew E Sloan, Manmeet S Ahluwalia, Justin D Lathia, Jennifer S Yu, Jeremy N Rich, Shideng Bao	EMBO Mol Med 12:e12291

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.