Human anti-cell free DNA ELISA Kit 60802

$570.00

Summary

ELISA kit for research use (RUO)
Reactivity: DNA
Suitable for: ELISA
Time:3 hours
Sample types: Serum, plasma, tissue homogenates and other biological fluids

SKU: 60802 Category: Indirect ELISA Tags: HCP, Human anti-cell free DNA, sandwich (sELISA)

Weight	1 lbs
Dimensions	9 × 5 × 2 in
target	Human anti-cell free DNA
species reactivity	human
applications	ELISA
assay type	Indirect & quantitative
available size	96 tests
sensitivity	0.938 ng/ml
range	1.563–100 ng/ml

Human anti-cell free DNA ELISA Kit

Assay type
ELISA

Research area
host cell protein

Sample type
Serum, plasma, tissue homogenates and other biological fluids

Components

Break apart microtiter test strips each coated single wells	8 x 12 (96 Total)
Lyophilized Standard	2 x vial
HRP Antibody(Concentrated, 100X)	120 uL
Washing solution concentrate (25X)	30 mL
Sample Dilution buffer	20 mL
Antibody Dilution buffer	10 mL
Stopping solution	10 mL
TMB Substrate (ready-to-use)	10 mL
Plate seals	3

Storage
Store at 2-8°C.

target relevance
DNA from host cell
Protein names Residual host cell DNA
Alternative names Host cell DNA, Residual DNA, Process-related DNA impurity
Description Residual genomic DNA originating from production host cells that may remain during manufacture of biologics, vaccines, and gene therapy products.

Data

Standard Curve

X axis: log | Y axis: linear

Standard	STD [ng/mL]	OD1	OD2	Average OD	Corrected OD
Blank #8	0	0.13	0.14	0.14	0
Standard #7	1.56	0.19	0.19	0.19	0.05
Standard #6	3.13	0.23	0.23	0.23	0.09
Standard #5	6.25	0.32	0.33	0.32	0.19
Standard #4	12.5	0.48	0.49	0.48	0.35
Standard #3	25	0.83	0.85	0.84	0.71
Standard #2	50	1.35	1.39	1.37	1.24
Standard #1	100	2.13	2.19	2.16	2.02

Precision

Sample	n	Mean	Standard Deviation	CV (%)
Intra Sample 1	20	3.2	0.16	4.96
Inter Sample 1	20	3.22	0.16	5.12
Intra Sample 2	20	13.1	0.61	4.69
Inter Sample 2	20	12.68	0.62	4.87
Intra Sample 3	20	48.66	2.64	5.43
Inter Sample 3	20	49.97	2.1	4.2

Recovery

Matrix	Recovery Range (%)	Average (%)
Serum(n=5)	91-102	99
EDTA Plasma(n=5)	90-105	97
HeparinPlasma(n=5)	85-102	92

Stability

37°C 1 Month (%)	2–8°C 6 Months (%)	2–8°C 12 Months (%)
80	95-100	85-98

Linearity

Sample	1:2 Dilution (%)	1:4 Dilution (%)	1:8 Dilution (%)
Serum(n=5)	86-103%	85-105%	94-101%
EDTAPlasma(n=5)	92-100%	83-101%	83-96%
Heparin Plasma(n=5)	82-96%	85-98%	87-100%

FAQ & Publications

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.