Immunohistochemistry (IHC) : Human urothelial carcinoma stained with anti-thymidylate synthase (TS) antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells.

rabbit anti-Thymidylate Synthase (TS) monoclonal antibody (ZR245) 6381

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to Thymidylate Synthase (TS)
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: 5-FU resistant cancer
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6381parent Category: antibody Tags: anatomy, General & Epithelial Markers, Thymidylate Synthase (TS)

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-Thymidylate Synthase (TS) monoclonal antibody ZR245 6381

antibody
Database link: human P04818
Tested applications IHC
Recommended dilutions Concentrated 1:50
Applicaiton Notes Positive control: 5-FU resistant cancer
Immunogen Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Thymidylate synthase (TS) (TSase) (EC 2.1.1.45)
Protein family Thymidylate synthase family
Mass 35716Da
Function Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Catalytic activity CATALYTIC ACTIVITY: Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP; Xref=Rhea:RHEA:12104, ChEBI:CHEBI:15636, ChEBI:CHEBI:57451, ChEBI:CHEBI:63528, ChEBI:CHEBI:246422; EC=2.1.1.45; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:12105; Evidence=;
Pathway PATHWAY: Pyrimidine metabolism; dTTP biosynthesis.
Subellular location Nucleus . Cytoplasm . Mitochondrion . Mitochondrion matrix . Mitochondrion inner membrane .
Structure Homodimer.
Involvement in disease Dyskeratosis congenita, digenic (DKCD) [MIM:620040]: A form of dyskeratosis congenita, a rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy. DKCD transmission pattern is consistent with digenic inheritance. Note=The disease is caused by variants affecting distinct genetic loci, including the gene represented in this entry. TYMS germline variants in the presence of a common ENOSF1 haplotype (defined by rs699517, rs2790 and rs1512643) result in severe thymidylate synthase deficiency and disease. The pathogenic mechanism involves increased expression of ENOSF1 relative to TYMS, and post-transcriptional inhibition of TYMS translation through ENOSF1-TYMS RNA-RNA interactions.
Target Relevance information above includes information from UniProt accession: P04818
The UniProt Consortium

Data

Human urothelial carcinoma stained with anti-thymidylate synthase (TS) antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.