Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human colon stained with clone ZR169 using peroxidase-conjugate and DAB chromogen. Note strong cytoplasmic staining of muscularis propria and weak staining of muscularis mucosae.

rabbit anti-Smoothelin monoclonal antibody (ZR169) 6436

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to Smoothelin
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Leiomyoma
Visualization: Cytoplasmic and nuclear
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6436parent Category: antibody Tags: anatomy, Smoothelin, Urinary Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-Smoothelin monoclonal antibody ZR169 6436

antibody
Database link: human O95425
Tested applications IHC
Recommended dilutions As directed
Immunogen Synthetic peptide corresponding to residues in human SDHB was used as an immunogen
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Supervillin (Archvillin) (p205/p250)
Protein family Villin/gelsolin family
Mass 247746Da
Function [Isoform 1]: Forms a high-affinity link between the actin cytoskeleton and the membrane. Is among the first costameric proteins to assemble during myogenesis and it contributes to myogenic membrane structure and differentiation (PubMed:12711699). Appears to be involved in myosin II assembly. May modulate myosin II regulation through MLCK during cell spreading, an initial step in cell migration. May play a role in invadopodial function (PubMed:19109420). .; [Isoform 2]: May be involved in modulation of focal adhesions. Supervillin-mediated down-regulation of focal adhesions involves binding to TRIP6. Plays a role in cytokinesis through KIF14 interaction (By similarity). .
Subellular location Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, invadopodium. Cell projection, podosome. Midbody . Cleavage furrow . Note=Tightly associated with both actin filaments and plasma membranes.
Tissues Expressed in many tissues. Most abundant in muscle, bone marrow, thyroid gland and salivary gland. Isoform 1 (archvillin) is muscle specific. .
Structure Associates with F-actin (PubMed:9867483). Interacts with NEB (PubMed:18639526). Interacts with MYH9 (By similarity). Interacts with MYLK (By similarity). Interacts with TASOR (By similarity). .; [Isoform 2]: Interacts with TRIP6 (By similarity). Interacts with DYNLT1 (By similarity). Interacts with KIF14; at midbody during cytokinesis (By similarity). .
Domain DOMAIN 2151..2214; /note="HP"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU00595"
Involvement in disease Myopathy, myofibrillar, 10 (MFM10) [MIM:619040]: A form of myofibrillar myopathy, a group of chronic neuromuscular disorders characterized at ultrastructural level by disintegration of the sarcomeric Z disk and myofibrils, and replacement of the normal myofibrillar markings by small dense granules, or larger hyaline masses, or amorphous material. MFM10 is an autosomal recessive disorder characterized by muscle pain, cramping, exercise fatigue, and progressive muscle rigidity. . Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: O95425
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded human colon stained with clone ZR169 using peroxidase-conjugate and DAB chromogen. Note strong cytoplasmic staining of muscularis propria and weak staining of muscularis mucosae.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.