Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded H&N invasive squamous cell carcinoma stained with anti-p16 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear and cytoplasmic staining of tumor cells

rabbit anti-p16INK4a monoclonal antibody (ZR407) 6306

Price range: $160.00 through $528.00

Antibody summary

Rabbit monoclonal to p16INK4a
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: HPV infected cervical squamous cell carcinoma
Visualization: Cytoplasmic and nuclear
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6306parent Category: antibody Tags: anatomy, Gynecological Pathology, monoclonal, p16INK4a

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-p16INK4a monoclonal antibody ZR407 6306

antibody
Database link: human P42771
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: HPV infected cervical squamous cell carcinoma
Immunogen Purified recombinant prokaryotic full length human p16INK4aprotein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Cyclin-dependent kinase inhibitor 2A (Cyclin-dependent kinase 4 inhibitor A) (CDK4I) (Multiple tumor suppressor 1) (MTS-1) (p16-INK4a) (p16-INK4) (p16INK4A)
Gene names CDKN2A,CDKN2A CDKN2 MTS1
Protein family CDKN2 cyclin-dependent kinase inhibitor family
Mass 16533Da
Function FUNCTION: Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. {ECO:0000269\|PubMed:16782892, ECO:0000269\|PubMed:7972006}.
Subellular location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269\|PubMed:17658461}. Nucleus {ECO:0000269\|PubMed:17658461}.
Tissues TISSUE SPECIFICITY: Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific. {ECO:0000269\|PubMed:10445844}.
Structure SUBUNIT: Heterodimer with CDK4 or CDK6. Predominant p16 complexes contained CDK6. Interacts with CDK4 (both 'T-172'-phosphorylated and non-phosphorylated forms); the interaction inhibits cyclin D-CDK4 kinase activity. Interacts with ISCO2. {ECO:0000269\|PubMed:16782892, ECO:0000269\|PubMed:17658461}.
Post-translational modification PTM: Phosphorylation seems to increase interaction with CDK4. {ECO:0000269\|PubMed:12529334}.
Involvement in disease DISEASE: Note=The association between cutaneous and uveal melanomas in some families suggests that mutations in CDKN2A may account for a proportion of uveal melanomas. However, CDKN2A mutations are rarely found in uveal melanoma patients.; DISEASE: Melanoma, cutaneous malignant 2 (CMM2) [MIM:155601]: A malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but may also involve other sites. {ECO:0000269\|PubMed:10651484, ECO:0000269\|PubMed:10874641, ECO:0000269\|PubMed:11506491, ECO:0000269\|PubMed:12019208, ECO:0000269\|PubMed:14646619, ECO:0000269\|PubMed:19260062, ECO:0000269\|PubMed:7987387, ECO:0000269\|PubMed:8595405, ECO:0000269\|PubMed:8653684, ECO:0000269\|PubMed:8710906, ECO:0000269\|PubMed:9328469, ECO:0000269\|PubMed:9425228}. Note=Disease susceptibility is associated with variants affecting the gene represented in this entry.; DISEASE: Familial atypical multiple mole melanoma-pancreatic carcinoma syndrome (FAMMMPC) [MIM:606719]: An inherited cancer predisposition syndrome characterized by an increased risk of developing malignant melanoma and/or pancreatic cancer. Mutation carriers within families may develop either or both types of cancer. Note=The disease is caused by variants affecting the gene represented in this entry.; DISEASE: Melanoma-astrocytoma syndrome (MASTS) [MIM:155755]: Characterized by a dual predisposition to melanoma and neural system tumors, commonly astrocytoma. {ECO:0000269\|PubMed:11136714}. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P42771
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded H&N invasive squamous cell carcinoma stained with anti-p16 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear and cytoplasmic staining of tumor cells

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.