rabbit anti-HNE polyclonal antibody 1007

Price range: $100.00 through $2,600.00

Antibody summary

Rabbit polyclonal to 4-Hydroxynonenal (4-HNE)
Suitable for: WB
Reacts with: HNE modified protein
Isotype: IgG
100 µg, 25 µg

available sizes

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SKU: 1007parent Categories: antibody, oxidative stress Tag: 4HNE

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	WB
reactivity	all
available sizes	1 mg, 100 µg, 25 µg

rabbit anti- hne polyclonal antibody 1007

antibody

Tested applications WB
Recommended dilutions Optimize
Immunogen 4-HNE-BC
Size and concentration 200µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer 75 mM Sodium Phosphate, 75 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH 7.2, 30% glycerol
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
HNE
Protein names 4-Hydroxynonenal
Alternative names 4-HNE, 4-Hydroxy-2-nonenal, HNE
Function 4-HNE acts as a bioactive lipid peroxidation product that modifies cellular signaling pathways, gene expression, inflammation, apoptosis, and oxidative stress responses.
Post-translational modification 4-HNE forms stable Michael addition and Schiff base adducts with cysteine, histidine, and lysine residues in proteins, producing 4-HNE-modified proteins that serve as markers of oxidative damage.
Involvement in disease Elevated levels of 4-HNE have been associated with atherosclerosis, cardiovascular disease, Alzheimer's disease, Parkinson's disease, diabetes, chronic inflammation, cancer, and aging.
Description 4-Hydroxynonenal (4-HNE) is a highly reactive alpha,beta-unsaturated aldehyde generated during lipid peroxidation of omega-6 polyunsaturated fatty acids. It is a major mediator and biomarker of oxidative stress and forms covalent adducts with proteins, lipids, and nucleic acids.
Chemical description Reactive lipid aldehyde C9H16O2 156.22 Da
Related entities relationship: derived_from; entity: Lipid peroxidation relationship: forms_adduct_with; entity: Proteins relationship: forms_adduct_with; entity: Low-density lipoprotein relationship: related_to; entity: Malondialdehyde relationship: related_to; entity: Oxidized LDL relationship: related_to; entity: 4-HNE-LDL
Keywords Reactive aldehyde, Lipid peroxidation, Oxidative stress, Protein modification, Oxidative damage biomarker
Structure 4-HNE is an alpha,beta-unsaturated hydroxyalkenal generated during oxidation of omega-6 polyunsaturated fatty acids.
Formation 4-HNE is produced during lipid peroxidation and accumulates under conditions of oxidative stress and inflammation.
Protein modification 4-HNE readily forms covalent adducts with cysteine, histidine, and lysine residues, creating stable 4-HNE-modified proteins and neoepitopes.
Clinical relevance Elevated 4-HNE levels are associated with cardiovascular disease, neurodegenerative disorders, diabetes, cancer, chronic inflammation, and aging.
Research relevance 4-HNE is widely used as a biomarker of lipid peroxidation and oxidative damage and is commonly detected by ELISA, immunohistochemistry, western blotting, mass spectrometry, and HPLC.

Data

Gel-image-anti-4-Hydroxynonenal (4-HNE) antibody-1007_1

Detection of 4-HNE by western blot. Samples: 1µg BSA conjugated to 4-HNE, Malondialdehyde (MDA), 4-Hydroxy-2-hexenal (4-HHE), Hexanoyl Lysine (H-Lys), and Crotonaldehyde (Croto). Antibody: Goat anti-4-HNE antibody (#1007) used at 1:1000. Secondary: HRP-conjugated donkey anti-goat IgG. Detection: Chemiluminescence with an exposure time of 30 seconds.

FAQ & Publications

Frequently Asked Questions

What is the recommended application for the rabbit anti-HNE polyclonal antibody 1007?

This antibody is suitable for use in Western blot (WB) applications.

How should the rabbit anti-HNE polyclonal antibody 1007 be stored to maintain its stability?

For short-term storage, keep the antibody at 2-8°C. For longer-term storage, it should be kept at -20°C, and freeze/thaw cycles should be avoided.

What immunogen was used to generate the rabbit anti-HNE polyclonal antibody 1007?

The antibody was raised against 4-HNE-BC as the immunogen.

Is the rabbit anti-HNE polyclonal antibody 1007 affinity purified and what is its isotype?

Yes, the antibody is affinity purified and its isotype is IgG.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.