rabbit anti-Ubiquitin polyclonal antibody 9044

$409.00

Antibody summary

Rabbit polyclonal to Ubiquitin
Suitable for: WB, ICC/IF, IHC
Reacts with: all
Isotype: IgG
100 µg

SKU: 9044 Categories: antibody, cell marker Tag: Ubiquitin

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
available sizes	100 µg

rabbit anti-Ubiquitin polyclonal antibody 9044

antibody
Database link: Ubiquitin Sequence - MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:5000-1:10000 IF/ICC and IHC: 1:500-1:1000
Immunogen Purified ubiquitin conjugated with glutaraldehyde to KLH
Size and concentration 100µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance

Ubiquitin plays a fundamental role in cell biology as a key regulator of protein degradation and cellular processes.

More on: cell markers and ubiquitin

Biotechnology
Ubiquitin, a small protein found in nearly all eukaryotic cells, plays a fundamental role in regulating various cellular processes. Its primary function is to mark proteins for degradation through the ubiquitin-proteasome system, a highly controlled and selective process known as ubiquitination. When a protein is destined for degradation or clearance, multiple ubiquitin molecules are covalently attached to specific lysine residues on the target protein, forming a polyubiquitin chain. This ubiquitin chain acts as a signal, directing the tagged protein to the proteasome, a large multi-subunit protein complex responsible for protein degradation. Once delivered to the proteasome, the ubiquitinated protein is unfolded and processed, and its constituent amino acids are recycled for the synthesis of new proteins. Apart from its role in protein degradation, ubiquitin also participates in various non-proteolytic functions, such as DNA repair, endocytosis, and signal transduction, where monoubiquitination or other ubiquitin modifications can serve as regulatory signals. The precise and intricate control of ubiquitin-mediated processes ensures proper cellular homeostasis, protein turnover, and the maintenance of critical cellular functions.

Data

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Detection of Ubiquitin by western blotting of Products from an Ubiquitination Assay and Purified Ubiquitin. Samples: A) Ubiquitination assay was performed as described in Zhang et al., 2004, Mol. Cell. Biol. 24(24):10941-10953. B) Purified ubiquitin applied in decreasing amounts from 5 to 0.1 µg. Antibody: Affinity purified rabbit anti-Ubiquitin antibody used at 0.4 µg/ml for western blot. Detection: Alkaline phosphatase conjugated secondary antibody and development with NBT/BCIP for 10 minutes (A and B).

benchmarkantibodies.com/wp-content/uploads/2024/03/9044_2.jpg

Detection of human and mouse Ubiquitin by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-Ubiquitin used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.