| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| available sizes | 1 mg, 100 µg |
rabbit anti-T7 tag polyclonal antibody 6777
$426.00 – $2,415.00
Antibody summary
- Rabbit polyclonal to T7 tag
- Suitable for: WB, ICC/IF, IP, ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG
- 100 µg, 1 mg
rabbit anti-T7 tag polyclonal antibody 6777
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:20,000. Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500 These are |
| Immunogen T7 (MASMTGGQQMG) conjugated to KLH. |
| Size and concentration 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS, pH 7.2, 0.09% NaN3. |
| Purity peptide affinty purifcation |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Biotechnology The T7 tag has emerged as a valuable tool in scientific research, particularly in molecular biology and protein expression studies. The T7 tag is a short peptide sequence (MASMTGGQQMG) derived from the T7 bacteriophage gene 10, which can be fused to the N or C terminus of a target protein. Its small size and minimal impact on the function of the tagged protein make it an attractive choice as a molecular tag. Researchers frequently use the T7 tag in protein expression systems, such as bacterial, insect, or mammalian cells, to facilitate the identification and purification of the recombinant protein. The T7 tag???s versatility allows for straightforward detection using specific T7 tag antibodies in various experimental assays, including Western blotting and immunoprecipitation. Moreover, the T7 tag can be employed for protein purification through affinity chromatography using T7 tag-specific resins, simplifying downstream characterization and functional studies. The widespread adoption of the T7 tag in research owes to its efficiency, reproducibility, and compatibility with a range of expression systems, making it an indispensable tool for investigating protein interactions, post-translational modifications, and protein localization in diverse biological contexts. |
Data
 |
| Detection of T7-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-T7 antibody 6777 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. |
|
| Detection of T7-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-T7 antibody 6777 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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