Name: rabbit anti-c-Jun (Phospho-Ser73) polyclonal antibody 1603
SKU: 1603parent
Price: 366.00 USD
Availability: InStock

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	c-Jun (Phospho-Ser73)
available sizes	100 µL

rabbit anti-c-Jun (Phospho-Ser73) polyclonal antibody 1603

antibody

Tested applications WB,IHC,IHC
Recommended dilutions Western blotting: use at dilution of 1:500- 1:1,000. A band of ~43kDa is detected. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications.
Immunogen Peptide sequence that includes phosphorylation site of Serine 73 (L-A-S(p)-P-E) derived from human c-Jun and conjugated to KLH.
Size and concentration 100µL and 1 mg/mL
Form liquid
Storage Instructions This antibody is stable for at least one (1) year at -20°C.
Storage buffer PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl,
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Transcription factor Jun (Activator protein 1) (AP1) (Proto-oncogene c-Jun) (Transcription factor AP-1 subunit Jun) (V-jun avian sarcoma virus 17 oncogene homolog) (p39)
Protein family BZIP family, Jun subfamily
Mass 35676Da
Function Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' (PubMed:10995748, PubMed:22083952). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity (By similarity). Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed:12618758). Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (PubMed:17210646). Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). {ECO:0000250\|UniProtKB:P05627, ECO:0000269\|PubMed:10995748, ECO:0000269\|PubMed:12618758, ECO:0000269\|PubMed:17210646, ECO:0000269\|PubMed:22083952, ECO:0000269\|PubMed:24623306}.; (Microbial infection) Upon Epstein-Barr virus (EBV) infection, binds to viral BZLF1 Z promoter and activates viral BZLF1 expression. {ECO:0000269\|PubMed:31341047}.
Subellular location Nucleus.
Tissues Expressed in the developing and adult prostate and prostate cancer cells. {ECO:0000269\|PubMed:24263171, ECO:0000269\|PubMed:24559171}.
Structure Heterodimer with either BATF3 or ATF7 (PubMed:10376527, PubMed:12087103, PubMed:15467742). Heterodimer with FOS (By similarity). Heterodimer with FOSB isoform 1 and 2 (By similarity). Component of an AP-1 transcription factor complex composed of JUN-FOS heterodimers (By similarity). As part of the AP-1 transcription factor complex, forms heterodimers with FOSB, thereby binding to the AP-1 consensus sequence and stimulating transcription (By similarity). Interacts with FOS and FOSB isoform 1 and 2 (By similarity). The ATF7/JUN heterodimer is essential for ATF7 transactivation activity (PubMed:10376527). Interacts with TSC22D3 (via N-terminus); the interaction inhibits the binding of active AP1 to its target DNA (By similarity). Interacts with HIVEP3 and MYBBP1A (By similarity). Interacts with SP1, SPIB and TCF20 (PubMed:10196196, PubMed:16478997, PubMed:8663478). Interacts with COPS5; the interaction leads indirectly to its phosphorylation (PubMed:8837781). Component of the SMAD3/SMAD4/JUN/FOS/complex which forms at the AP1 promoter site (PubMed:10995748). The SMAD3/SMAD4 heterodimer acts synergistically with the JUN/FOS heterodimer to activate transcription in response to TGF-beta (PubMed:9732876). Interacts (via its basic DNA binding and leucine zipper domains) with SMAD3 (via an N-terminal domain); the interaction is required for TGF-beta-mediated transactivation of the SMAD3/SMAD4/JUN/FOS/complex (PubMed:10995748). Interacts with methylated RNF187 (PubMed:20852630, PubMed:23624934). Binds to HIPK3. Interacts (when phosphorylated) with FBXW7 (PubMed:14739463). Found in a complex with PRR7 and FBXW7 (PubMed:27458189). Interacts with PRR7 and FBXW7; the interaction inhibits ubiquitination-mediated JUN degradation promoting its phosphorylation and transcriptional activity (PubMed:27458189). Interacts with RBM39 (By similarity). Interacts with PAGE4 (PubMed:24263171, PubMed:24559171, PubMed:26242913). Interacts with ARK2N and CSNK2B; the interaction with ARK2N is mediated by CSNK2B (PubMed:31341047). {ECO:0000250\|UniProtKB:P05627, ECO:0000250\|UniProtKB:P17325, ECO:0000269\|PubMed:10196196, ECO:0000269\|PubMed:10376527, ECO:0000269\|PubMed:10995748, ECO:0000269\|PubMed:12087103, ECO:0000269\|PubMed:14739463, ECO:0000269\|PubMed:15467742, ECO:0000269\|PubMed:16478997, ECO:0000269\|PubMed:20852630, ECO:0000269\|PubMed:23624934, ECO:0000269\|PubMed:24263171, ECO:0000269\|PubMed:24559171, ECO:0000269\|PubMed:26242913, ECO:0000269\|PubMed:27458189, ECO:0000269\|PubMed:31341047, ECO:0000269\|PubMed:8663478, ECO:0000269\|PubMed:8837781, ECO:0000269\|PubMed:9732876}.
Post-translational modification Ubiquitinated by the SCF(FBXW7), leading to its degradation (PubMed:14739463, PubMed:27458189). Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7 (PubMed:14739463). {ECO:0000269\|PubMed:14739463, ECO:0000269\|PubMed:27458189}.; Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity. Phosphorylated by VRK1 (PubMed:31527692). {ECO:0000269\|PubMed:14739463, ECO:0000269\|PubMed:17210646, ECO:0000269\|PubMed:17804415, ECO:0000269\|PubMed:1846781, ECO:0000269\|PubMed:18650425, ECO:0000269\|PubMed:21177766, ECO:0000269\|PubMed:22307329, ECO:0000269\|PubMed:31527692, ECO:0000269\|PubMed:8464713, ECO:0000269\|PubMed:8855261}.; Acetylated at Lys-271 by EP300. {ECO:0000269\|PubMed:11689449}.
Target Relevance information above includes information from UniProt accession: P05412
The UniProt Consortium

Data

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Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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