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mouse anti-Myoferlin monoclonal antibody (7D2) 2343

$520.00

Antibody summary

  • Mouse monoclonal to Myoferlin
  • Suitable for: WB,ICC
  • Isotype: IgG2a
  • 100 µg
SKU: 2343parent Category: Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG2a

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Myoferlin

available sizes

100 µg

mouse anti-Myoferlin monoclonal antibody (7D2) 2343

antibody
Tested applications
WB,ICC/IF
Recommended dilutions
Immunoblotting: use at 1-10ug/mL. A band of ~230kDa is detected.

Immunocytochemistry: use at 1- 20ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
Immunogen
Synthetic peptide derived from the N-terminal domain of the human FER1L3 protein. Accession no. Q9NZM1.
Size and concentration
100µg and
Form
lyophilized
Storage Instructions
This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Storage buffer
Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose
Purity
protein affinty purification
Clonality
monoclonal
Isotype
IgG2a
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG2a - Isotype Control
target relevance
Protein names
Myoferlin (Fer-1-like protein 3)
Gene names
MYOF,MYOF FER1L3 KIAA1207
Protein family
Ferlin family
Mass
234709Da
Function
Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR (By similarity).
Subellular location
Cell membrane; Single-pass type II membrane protein. Nucleus membrane; Single-pass type II membrane protein. Cytoplasmic vesicle membrane; Single-pass type II membrane protein. Note=Concentrated at the membrane sites of both myoblast-myoblast and myoblast-myotube fusions. Detected at the plasmalemma in endothelial cells lining intact blood vessels (By similarity). Found at nuclear and plasma membranes. Enriched in undifferentiated myoblasts near the plasma membrane in puncate structures.
Tissues
Expressed in myoblast and endothelial cells (at protein level). Highly expressed in cardiac and skeletal muscles. Also present in lung, and at very low levels in kidney, placenta and brain.
Structure
Interacts with DNM2 and KDR. Interacts with EHD1 (By similarity). Interacts with EHD2; the interaction is direct (PubMed:18502764). Interacts with RIPOR2 (PubMed:24687993).
Domain
TOPO_DOM 1
Involvement in disease
DISEASE: Angioedema, hereditary, 7 (HAE7) [MIM:619366]: A form of angioedema, a disorder characterized by episodic local swelling involving subcutaneous or submucous tissue of the upper respiratory and gastrointestinal tracts, face, extremities, and genitalia. HAE7 is an autosomal dominant form characterized by onset of recurrent swelling of the face, lips, and oral mucosa in the second decade. Note=The disease may be caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: Q9NZM1
The UniProt Consortium

Data

benchmark-antibodies_anti-myoferlin_antibody_2343_1.jpg
Immunoblotting: use at 1-10ug/ml. A band of ~230kDa is detected. Detection of myoferlin in (1) C2C12 cell lysate and (2) human fibroblast cell lysate with #2343.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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