| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| reactivity | VSV-G |
| available sizes | 100 µg |
rabbit anti-VSV-G polyclonal antibody 1440
$426.00
Antibody summary
- Rabbit polyclonal to VSV-G
- Suitable for: ELISA,ICC,IHC,WB
- Isotype: IgG
- 100 µg
rabbit anti-VSV-G polyclonal antibody 1440
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:20,000. Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate. ELISA: for detection use at a dilution of 1:1,000-1:30,000 These are recommended dilutions. Endusers shoul |
| Immunogen Synthetic peptide YTDIEMNRLGK conjugated to KLH. |
| Size and concentration 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS, pH 7.2, 0.09% NaN3. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Vesicular stomatitis virus glycoprotein tag, VSV-G epitope |
| Biotechnology The VSV-G tag has become a valuable and versatile tool in scientific research, particularly in molecular biology, cell biology, and virology studies. The VSV-G tag is derived from the glycoprotein of the Vesicular Stomatitis Virus (VSV), a virus that infects animals and does not replicate in mammalian cells. This feature makes the VSV-G tag highly useful for pseudotyping viral particles and creating viral vectors for gene delivery and expression studies. Researchers frequently use the VSV-G tag to replace the native envelope protein of viral vectors, enabling efficient and broad transduction of target cells, including difficult-to-transfect cell types. Furthermore, the VSV-G tag is often employed as a fusion tag for protein expression and purification, as it is well-tolerated and effectively trafficked to the plasma membrane. The VSV-G tag can be readily detected using specific antibodies in a variety of experimental assays, such as Western blotting and immunofluorescence, facilitating the visualization and quantification of tagged proteins. The widespread application of the VSV-G tag in research can be attributed to its utility in viral vector design, as well as its compatibility with multiple expression systems, making it a valuable tool for investigating cellular processes, gene function, and protein dynamics in diverse biological contexts. |
Data
 |
| Detection of VSV-G-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-VSV-G antibody 1440 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
|
| Detection of VSV-G-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-VSV-G antibody 1440 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| # | SDS | Certificate | |
|---|---|---|---|
| Please enter your product and batch number here to retrieve product datasheet, SDS, and QC information. | |||
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