| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| reactivity | VSV-G |
| available sizes | 100 µg |
rabbit anti-VSV-G polyclonal antibody 1440
$426.00
Antibody summary
- Rabbit polyclonal to VSV-G
- Suitable for: ELISA,ICC,IHC,WB
- Isotype: IgG
- 100 µg
rabbit anti-VSV-G polyclonal antibody 1440
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:20,000. Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate. ELISA: for detection use at a dilution of 1:1,000-1:30,000 These are recommended dilutions. Endusers shoul |
| Immunogen Synthetic peptide YTDIEMNRLGK conjugated to KLH. |
| Size and concentration 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS, pH 7.2, 0.09% NaN3. |
| Purity peptide affinty purifcation |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Biotechnology The VSV-G tag has become a valuable and versatile tool in scientific research, particularly in molecular biology, cell biology, and virology studies. The VSV-G tag is derived from the glycoprotein of the Vesicular Stomatitis Virus (VSV), a virus that infects animals and does not replicate in mammalian cells. This feature makes the VSV-G tag highly useful for pseudotyping viral particles and creating viral vectors for gene delivery and expression studies. Researchers frequently use the VSV-G tag to replace the native envelope protein of viral vectors, enabling efficient and broad transduction of target cells, including difficult-to-transfect cell types. Furthermore, the VSV-G tag is often employed as a fusion tag for protein expression and purification, as it is well-tolerated and effectively trafficked to the plasma membrane. The VSV-G tag can be readily detected using specific antibodies in a variety of experimental assays, such as Western blotting and immunofluorescence, facilitating the visualization and quantification of tagged proteins. The widespread application of the VSV-G tag in research can be attributed to its utility in viral vector design, as well as its compatibility with multiple expression systems, making it a valuable tool for investigating cellular processes, gene function, and protein dynamics in diverse biological contexts. |
Data
 |
| Detection of VSV-G-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-VSV-G antibody 1440 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
|
| Detection of VSV-G-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-VSV-G antibody 1440 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
|---|
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| # | |||
|---|---|---|---|
| Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information. | |||
Only logged in customers who have purchased this product may leave a review.
Reviews
There are no reviews yet.