| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| available sizes | 100 µg |
rabbit anti-V5 tag polyclonal antibody 3621
$418.00
Antibody summary
- Rabbit polyclonal to V5 tag
- Suitable for: WB, ICC/IF, IP, ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG
- 100 µg
rabbit anti-V5 tag polyclonal antibody 3621
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:20,000. Immunocytochemistry: use at a dilution of 1:100-1:400. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500 These are recommended dilutions. Endusers should det |
| Immunogen Synthetic peptide corresponding to aa 95-108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian virus 5 conjugated to KLH. |
| Size and concentration 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS, pH 7.2, 0.09% NaN3. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| V5 tag |
| Protein names V5 epitope tag |
| Alternative names V5 tag, V5 epitope, SV5 tag, Pk tag |
| Function The V5 tag is an artificial peptide epitope used for the detection, purification, and characterization of recombinant proteins. It is recognized by anti-V5 antibodies in applications including western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, and immunoprecipitation. |
| Description The V5 tag is a synthetic peptide epitope derived from the P and V proteins of Human orthorubulavirus 5 (formerly Simian Virus 5, SV5). It is commonly fused to recombinant proteins for detection, purification, and localization studies. |
| Keywords Epitope tag, Protein detection, Protein purification, Recombinant protein |
| Sequence GKPIPNPLLGLDST |
| Structure The V5 tag is a 14-amino acid peptide epitope with the sequence GKPIPNPLLGLDST derived from the P and V proteins of Human orthorubulavirus 5 (formerly SV5). |
| Function The V5 tag is used as a molecular tag for the detection, purification, and localization of recombinant proteins. |
| Applications V5-tagged proteins can be detected by western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, and immunoprecipitation. |
| Research relevance The V5 tag is widely used in recombinant protein expression systems and serves as an alternative to FLAG, HA, Myc, and His tags. |
Data
 |
| Detection of V5-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-V5 antibody 3621 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
|
| Detection of V5-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-V5 antibody 3621 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
FAQ & Publications
Frequently Asked Questions
What are the recommended applications for the rabbit anti-V5 tag polyclonal antibody 3621?
This antibody is suitable for Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), immunoprecipitation (IP), and ELISA.
How should the rabbit anti-V5 tag polyclonal antibody be stored to maintain stability?
The antibody should be stored at 2-8°C, where it remains stable for at least one year.
What is the immunogen used to generate the rabbit anti-V5 tag polyclonal antibody 3621?
The immunogen is a synthetic peptide corresponding to amino acids 95-108 (GKPIPNPLLGLDST) of the RNA polymerase alpha subunit of simian virus 5, conjugated to KLH.
What are the recommended dilution ranges for using this antibody in Western blot and ELISA assays?
For Western blotting, use a dilution range of 1:1,000 to 1:20,000. For ELISA detection, use 1:1,000 to 1:30,000; for ELISA coating, use 1:100 to 1:500.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
Only logged in customers who have purchased this product may leave a review.
Reviews
There are no reviews yet.