| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | V5 |
| available sizes | 100 µg |
rabbit anti-V5 HRP polyclonal antibody 6149
$513.00
Antibody summary
- Rabbit polyclonal to V5 , HRP conjugate
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 100 µg
rabbit anti-V5 HRP polyclonal antibody 6149
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:30,000. Immunocytochemistry: use at a dilution of 1:200-1:500 ELISA: use at a dilution of 1:10,000- 1:100,000 These are recommended dilutions. Endusers should determine optimal dilutions for their applications. |
| Immunogen Synthetic peptide corresponding to aa 95-108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian virus 5 conjugated to KLH. |
| Size and concentration 100µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.2% |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| V5 tag |
| Protein names V5 epitope tag |
| Alternative names V5 tag, V5 epitope, SV5 tag, Pk tag |
| Function The V5 tag is an artificial peptide epitope used for the detection, purification, and characterization of recombinant proteins. It is recognized by anti-V5 antibodies in applications including western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, and immunoprecipitation. |
| Description The V5 tag is a synthetic peptide epitope derived from the P and V proteins of Human orthorubulavirus 5 (formerly Simian Virus 5, SV5). It is commonly fused to recombinant proteins for detection, purification, and localization studies. |
| Keywords Epitope tag, Protein detection, Protein purification, Recombinant protein |
| Sequence GKPIPNPLLGLDST |
| Structure The V5 tag is a 14-amino acid peptide epitope with the sequence GKPIPNPLLGLDST derived from the P and V proteins of Human orthorubulavirus 5 (formerly SV5). |
| Function The V5 tag is used as a molecular tag for the detection, purification, and localization of recombinant proteins. |
| Applications V5-tagged proteins can be detected by western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, and immunoprecipitation. |
| Research relevance The V5 tag is widely used in recombinant protein expression systems and serves as an alternative to FLAG, HA, Myc, and His tags. |
Data
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| ROR1 and ROR2 protein expression as measured by immunohistochemistry. Representative images of score 0 (absence), 1 (weak), 2 (moderate), 3 (intense) for both ROR1 and ROR2. |
 |
| ROR1 knockdown and ROR2 overexpression significantly decreased proliferation and migration of KLE. (-) ROR1 mRNA expression level was reduced significantly without changing ROR2 following single ROR1 siRNA transfection. ROR2 mRNA expression level was elevated significantly with no changes in ROR1 mRNA level following single ROR2 plasmid transfection. Cotransfecting ROR1 siRNA and ROR2 plasmid significantly reduced ROR1 while increased ROR2 at mRNA level. (-) Representative western blot membranes showed effective delivery of ROR1 siRNA and/or ROR2 plasmid in KLE. (-) ROR1 knockdown and ROR2 overexpression significantly reduced the cell proliferation after 48h and 72h (p=0.043 and 0.004 respectively). (-): ROR1 knockdown and/or ROR2 overexpression had no effect on adhesion to collagen or fibronectin. (-): ROR1 knockdown and ROR2 overexpression decreased KLE migration ability significantly (p=0.037). (-) No significant change was observed for invasion following ROR1 knockdown and/or ROR2 overexpression. For all panels n=3, error bars represent standard deviation of the mean, *p<0.05. |
 |
| ROR1 overexpression and ROR2 knockdown play different roles in MFE-296. (-) ROR2 mRNA level was reduced significantly without changing ROR1 following single ROR2 siRNA transfection. ROR1 mRNA level was increased significantly with no change in ROR2 following single ROR1 plasmid transfection. Cotransfecting ROR2 siRNA and ROR1 plasmid significantly reduced ROR2 while increased ROR1 at mRNA level. (-) Representative western blot membranes showed effective delivery of ROR2 siRNA and/or ROR1 plasmid in MFE-296. (-) No significant change of proliferation was observed after 48h or 72h following ROR1 overexpression and/or ROR2 knockdown. (-) ROR2 knockdown and/or ROR1 overexpression had no effect on adhesion to collagen or fibronectin. (-) ROR1 knockdown and/or ROR2 overexpression did not change MFE-296 cell migration significantly. (-) No significant change was observed for invasion following ROR2 knockdown and/or ROR1 overexpression. For all panels n=3, error bars represent standard deviation of the mean, *Significant at p<0.05. |
 |
| Detection of V5-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, rabbit anti-V5 antibody 6149 used for WB at 0.2 µg /ml (1:5,000). Detection: Chemiluminescence with an exposure time of 30 seconds. |
FAQ & Publications
Frequently Asked Questions
What applications is the rabbit anti-V5 HRP polyclonal antibody 6149 validated for, and what are the recommended dilutions?
This antibody is validated for Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA. Recommended dilutions are 1:1,000 to 1:30,000 for immunoblotting, 1:200 to 1:500 for immunocytochemistry, and 1:10,000 to 1:100,000 for ELISA. End users should optimize dilutions for their specific applications.
How should the rabbit anti-V5 HRP polyclonal antibody 6149 be stored to maintain its stability?
The antibody should be stored at 2-8°C, where it remains stable for at least one year. It is provided in a phosphate buffered saline (PBS) solution containing 0.2% as a stabilizer.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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