| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | TRAIL (CT) |
| available sizes | 100 µg |
rabbit anti-TRAIL (CT) polyclonal antibody 3539
$445.00
Antibody summary
- Rabbit polyclonal to TRAIL (CT)
- Suitable for: ELISA,WB,IHC-P,IF
- Isotype: IgG
- 100 µg
rabbit anti-TRAIL (CT) polyclonal antibody 3539
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at 1ug/mL. Positive control: HeLa cell lysate. Immunocytochemistry: use at 20ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their application. |
| Immunogen Synthetic peptide corresponding to aa 261-277 of human TRAIL (accession no. NP_003801). |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity immunogen affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Homo sapiens TNFSF10 Tumor necrosis factor ligand superfamily member 10 |
| Protein names Tumor necrosis factor ligand superfamily member 10 |
| Alternative names Apo-2 ligand, TNF-related apoptosis-inducing ligand |
| Gene names TNFSF10 |
| Protein family Belongs to the tumor necrosis factor family |
| Function Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed:10549288, PubMed:26457518). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis |
| Subcellular location Cell membrane, Secreted |
| Structure Homotrimer (PubMed:26457518). One TNFSF10 homotrimer interacts with three TNFSF10A mononers (PubMed:26457518). One TNFSF10 homotrimer interacts with three TNFSF10B mononers (PubMed:10549288) |
| Post-translational modification Tyrosine phosphorylated by PKDCC/VLK |
| Keywords 3D-structure, Alternative splicing, Apoptosis, Cell membrane, Cytokine, Membrane, Metal-binding, Phosphoprotein, Proteomics identification, Reference proteome, Secreted, Signal-anchor, Transmembrane, Transmembrane helix, Zinc |
| Sequence MAMMEVQGGPSLGQTCVLIVIFTVLLQSLCVAVTYVYFTNELKQMQDKYSKSGIACFLKE DDSYWDPNDEESMNSPCWQVKWQLRQLVRKMILRTSEETISTVQEKQQNISPLVRERGPQ RVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKG FYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLY SIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG |
| UniProt accession: P50591 |
Data
 |
| Western blot analysis of TRAIL in HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with TRAIL antibody at 1 µg/mL. |
 |
| Immunohistochemistry of TRAIL in human brain tissue with TRAIL antibody at 20 µg/mL. |
 |
| Western blot analysis of TRAIL in human brain tissue lysate with TRAIL antibody at (A) 2.5 and (B) 5 µg/mL. |
 |
| Immunofluorescence of TRAIL in human brain tissue with Trail antibody at 20 µg/mL.
Red: Trail Antibody (3539) Blue: DAPI staining |
FAQ & Publications
Frequently Asked Questions
What applications has the rabbit anti-TRAIL (CT) polyclonal antibody 3539 been validated for?
This antibody is suitable for ELISA, Western blotting (WB), immunohistochemistry on paraffin-embedded tissues (IHC-P), and immunofluorescence (IF). It has been tested in ICC/IF and WB applications specifically.
How should I store the rabbit anti-TRAIL (CT) polyclonal antibody to maintain its stability?
The antibody should be stored at -20°C where it remains stable for at least one year. It is important to avoid multiple freeze-thaw cycles to preserve antibody integrity.
What is the immunogen used to generate the rabbit anti-TRAIL (CT) polyclonal antibody 3539?
The immunogen is a synthetic peptide corresponding to amino acids 261-277 of the human TRAIL protein (accession number NP_003801).
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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