| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | TRAIL (CT) |
| available sizes | 100 µg |
rabbit anti-TRAIL (CT) polyclonal antibody 3539
$445.00
Antibody summary
- Rabbit polyclonal to TRAIL (CT)
- Suitable for: ELISA,WB,IHC-P,IF
- Isotype: IgG
- 100 µg
rabbit anti-TRAIL (CT) polyclonal antibody 3539
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at 1ug/mL. Positive control: HeLa cell lysate. Immunocytochemistry: use at 20ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their application. |
| Immunogen Synthetic peptide corresponding to aa 261-277 of human TRAIL (accession no. NP_003801). |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity immunogen affinty purifcation |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Tumor necrosis factor ligand superfamily member 10 (Apo-2 ligand) (Apo-2L) (TNF-related apoptosis-inducing ligand) (Protein TRAIL) (CD antigen CD253) |
| Protein family Tumor necrosis factor family |
| Mass 32509Da |
| Function Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed:10549288, PubMed:26457518). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. {ECO:0000269|PubMed:10549288, ECO:0000269|PubMed:26457518}. |
| Subellular location Cell membrane {ECO:0000269|PubMed:14609566}; Single-pass type II membrane protein {ECO:0000305}. Secreted {ECO:0000269|PubMed:14609566}. Note=Exists both as membrane-bound and soluble form. {ECO:0000269|PubMed:14609566}. |
| Tissues Widespread; most predominant in spleen, lung and prostate. |
| Structure Homotrimer (PubMed:26457518). One TNFSF10 homotrimer interacts with three TNFSF10A mononers (PubMed:26457518). One TNFSF10 homotrimer interacts with three TNFSF10B mononers (PubMed:10549288). {ECO:0000269|PubMed:10549288, ECO:0000269|PubMed:26457518}. |
| Post-translational modification Tyrosine phosphorylated by PKDCC/VLK. {ECO:0000269|PubMed:25171405}. |
| Target Relevance information above includes information from UniProt accession: P50591 |
| The UniProt Consortium |
 |
| Western blot analysis of TRAIL in HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with TRAIL antibody at 1 µg/mL. |
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| Immunohistochemistry of TRAIL in human brain tissue with TRAIL antibody at 20 µg/mL. |
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| Western blot analysis of TRAIL in human brain tissue lysate with TRAIL antibody at (A) 2.5 and (B) 5 µg/mL. |
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| Immunofluorescence of TRAIL in human brain tissue with Trail antibody at 20 µg/mL.
Red: Trail Antibody (3539) Blue: DAPI staining |
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
| relevant to this product |
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| Western blot IHC ICC |
| # | SDS | Certificate | |
|---|---|---|---|
| Please enter your product and batch number here to retrieve product datasheet, SDS, and QC information. | |||
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