| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| available sizes | 100 µg |
rabbit anti-T7 tag polyclonal antibody 6777
$426.00
Antibody summary
- Rabbit polyclonal to T7 tag
- Suitable for: WB, ICC/IF, IP, ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG
- 100 µg
rabbit anti-T7 tag polyclonal antibody 6777
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:20,000. Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500 These are |
| Immunogen T7 (MASMTGGQQMG) conjugated to KLH. |
| Size and concentration 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS, pH 7.2, 0.09% NaN3. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| T7 tag |
| Protein names T7 epitope tag |
| Alternative names T7 tag, T7 epitope, Gene 10 tag, MASMTGGQQMG tag |
| Function The T7 tag is an artificial peptide epitope used for detection and characterization of recombinant proteins using anti-T7 tag antibodies. |
| Description The T7 tag is a peptide epitope derived from the major capsid protein (gene 10 protein) of bacteriophage T7. It is commonly used as a recombinant protein tag for detection, purification, and characterization of fusion proteins. |
| Keywords Epitope tag, Protein detection, Protein purification, Immunoprecipitation, Recombinant protein |
| Sequence MASMTGGQQMG |
| Structure The T7 tag is an 11-amino acid peptide epitope with the sequence MASMTGGQQMG derived from the bacteriophage T7 gene 10 major capsid protein. |
| Function The T7 tag is used as a molecular epitope tag for detection, purification, and characterization of recombinant proteins. |
| Detection T7-tagged proteins can be detected using anti-T7 tag antibodies in western blotting, ELISA, immunofluorescence, immunocytochemistry, and immunoprecipitation assays. |
| Applications The T7 tag is commonly incorporated into expression vectors and fused to recombinant proteins to facilitate detection and purification. |
| Research relevance The T7 tag is widely used in bacterial expression systems and molecular biology workflows and serves as an alternative to FLAG, HA, Myc, V5, and His tags. |
Data
 |
| Detection of T7-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-T7 antibody 6777 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. |
|
| Detection of T7-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-T7 antibody 6777 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. |
FAQ & Publications
Frequently Asked Questions
What experimental applications is the rabbit anti-T7 tag polyclonal antibody 6777 validated for?
This antibody is validated for use in Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), immunoprecipitation (IP), and ELISA assays.
What is the recommended dilution range for using this antibody in Western blotting?
For immunoblotting, it is recommended to use the antibody at a dilution between 1:1,000 and 1:20,000.
How should the rabbit anti-T7 tag polyclonal antibody 6777 be stored to maintain stability?
The antibody is stable for at least one year when stored at 2-8°C in its supplied storage buffer (PBS, pH 7.2, containing 0.09% sodium azide).
What is the host species and clonality of this anti-T7 tag antibody?
The antibody is a rabbit polyclonal IgG.
What is the immunogen used to generate this rabbit anti-T7 tag polyclonal antibody?
The immunogen is the T7 peptide sequence (MASMTGGQQMG) conjugated to keyhole limpet hemocyanin (KLH).
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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