| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | STAT5A (phospho-Tyr694) |
| available sizes | 100 µL |
rabbit anti-STAT5A (phospho-Tyr694) polyclonal antibody 9804
$366.00
Antibody summary
- Rabbit polyclonal to STAT5A (phospho-Tyr694)
- Suitable for: WB,IHC
- Isotype: Whole IgG
- 100 µl
rabbit anti-STAT5A (phospho-Tyr694) polyclonal antibody 9804
| antibody |
|---|
| Tested applications WB,IHC,IHC |
| Recommended dilutions Immunoblotting: use at dilution of 1:500-1:1,000. A band of~90kDa is detected. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications. |
| Immunogen Peptide sequence that includes phosphorylation site of tyrosine 694 (D-G-Y(p)-V-K) derived from human STAT5A and conjugated to KLH. |
| Size and concentration 100µL and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. |
| Storage buffer PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Signal transducer and activator of transcription 5A |
| Gene names STAT5A,STAT5A STAT5 |
| Protein family Transcription factor STAT family |
| Mass 90647Da |
| Function FUNCTION: Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. {ECO:0000269|PubMed:15534001}. |
| Subellular location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15534001}. Nucleus {ECO:0000269|PubMed:15534001}. Note=Translocated into the nucleus in response to phosphorylation. |
| Structure SUBUNIT: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7 (PubMed:12954634). Interacts with ERBB4 (PubMed:15534001). Interacts with EBF4 (PubMed:35939714). Interacts with CD69 (By similarity). {ECO:0000250|UniProtKB:P42230, ECO:0000269|PubMed:12954634, ECO:0000269|PubMed:15534001, ECO:0000269|PubMed:35939714}. |
| Post-translational modification PTM: Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO (By similarity). Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus (PubMed:21135090). Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity (By similarity). Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3 (PubMed:14504097). Alternatively, can be phosphorylated by JAK2 at Tyr-694 (PubMed:12529425). {ECO:0000250|UniProtKB:P40763, ECO:0000250|UniProtKB:P42230, ECO:0000250|UniProtKB:Q62771, ECO:0000269|PubMed:12529425, ECO:0000269|PubMed:14504097, ECO:0000269|PubMed:21135090}.; PTM: ISGylated. {ECO:0000250|UniProtKB:P42230}. |
| Target Relevance information above includes information from UniProt accession: P42229 |
| The UniProt Consortium |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What are the recommended applications and dilution ranges for using the rabbit anti-STAT5A (phospho-Tyr694) polyclonal antibody 9804?
This antibody is suitable for Western blotting (WB) and immunohistochemistry (IHC). For immunoblotting, the recommended dilution is 1:500 to 1:1,000, where a band of approximately 90 kDa is detected. For immunohistochemistry, use a dilution range of 1:50 to 1:100. Users should optimize dilutions for their specific experimental conditions.
How should the rabbit anti-STAT5A (phospho-Tyr694) polyclonal antibody 9804 be stored to maintain its stability?
The antibody is supplied as a liquid at a concentration of 1 mg/mL in PBS buffer without magnesium and calcium ions, pH 7.4, containing 150 mM NaCl. It should be stored at -20°C and is stable for at least one year under these conditions.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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