| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Rel (Phospho-Ser503) |
| available sizes | 100 µL |
rabbit anti-Rel (Phospho-Ser503) polyclonal antibody 5864
$366.00
Antibody summary
- Rabbit polyclonal to Rel (Phospho-Ser503)
- Suitable for: IF
- Isotype: Whole IgG
- 100 µl
rabbit anti-Rel (Phospho-Ser503) polyclonal antibody 5864
| antibody |
|---|
| Tested applications #VALUE! |
| Recommended dilutions Immunofluorescence: use at dilution of 1:100-1:200. These are recommended working dilutions. End user should determine optimal dilutions for their applications. |
| Immunogen Peptide sequence that includes phosphorylation site of Serine 503 (T-S-S(p)-D-S) derived from human Rel and conjugated to KLH. |
| Size and concentration 100µL and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. |
| Storage buffer PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Proto-oncogene c-Rel |
| Gene names REL,REL |
| Mass 68520Da |
| Function FUNCTION: Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator. |
| Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. |
| Structure SUBUNIT: Component of the NF-kappa-B p65-c-Rel complex. Component of the NF-kappa-B p50-c-Rel complex. Component of the NF-kappa-B p52-c-Rel complex. Homodimer; component of the NF-kappa-B c-Rel-c-Rel complex (By similarity). Interacts with NKIRAS1. Interacts with NFKBIB (By similarity). Interacts with NFKBIE. {ECO:0000250, ECO:0000269|PubMed:15024091, ECO:0000269|PubMed:15102766, ECO:0000269|PubMed:1740106, ECO:0000269|PubMed:8152812, ECO:0000269|PubMed:9056676, ECO:0000269|PubMed:9315679}. |
| Involvement in disease DISEASE: Immunodeficiency 92 (IMD92) [MIM:619652]: An autosomal recessive disorder characterized by recurrent bacterial, viral, fungal, or parasitic infections appearing in infancy or early childhood. Patient lymphocytes show defects in both T- and B-cell proliferation, cytokine secretion, and overall function, and there is also evidence of dysfunction of NK, certain antigen-presenting cells, and myeloid subsets. {ECO:0000269|PubMed:31103457, ECO:0000269|PubMed:34623332}. Note=The disease is caused by variants affecting the gene represented in this entry. |
| Target Relevance information above includes information from UniProt accession: Q04864 |
| The UniProt Consortium |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What applications has the rabbit anti-Rel (Phospho-Ser503) polyclonal antibody 5864 been validated for?
This antibody has been tested and is suitable for immunofluorescence (IF) and western blot (WB) applications.
How should the rabbit anti-Rel (Phospho-Ser503) antibody 5864 be stored to maintain stability?
The antibody should be stored at -20°C, where it remains stable for at least one year.
What is the recommended dilution range for using this antibody in immunofluorescence?
For immunofluorescence, the recommended working dilution is between 1:100 and 1:200, although end users should optimize this for their specific applications.
What is the immunogen used to generate the rabbit anti-Rel (Phospho-Ser503) polyclonal antibody 5864?
The immunogen is a peptide sequence containing the phosphorylation site at Serine 503 (T-S-S(p)-D-S) derived from human Rel protein and conjugated to KLH.
Which secondary antibodies are compatible with the rabbit anti-Rel (Phospho-Ser503) polyclonal antibody?
Compatible secondary antibodies include goat anti-rabbit IgG, H&L chain specific antibodies conjugated to peroxidase, biotin, or FITC, including cross-absorbed versions for improved specificity.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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