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rabbit anti-Raidd (CT) polyclonal antibody 8020

$445.00

Antibody summary

  • Rabbit polyclonal to Raidd (CT)
  • Suitable for: ELISA,WB,IHC-P,ICC,IF
  • Isotype: IgG
  • 100 µg
SKU: 8020parent Category: Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

RAIDD (CT)

available sizes

100 µg

rabbit anti-Raidd (CT) polyclonal antibody 8020

antibody
Tested applications
WB,IHC,IHC,ICC/IF,ELISA
Recommended dilutions
Immunoblotting : use at 1ug/mL.

Positive control: HeLa cell lysate.

Immunohistochemistry: use at 10ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for ther applications.
Immunogen
Synthetic peptide corresponding to aa 181-199 at the C-terminus of human RAIDD (accession no. AAB42217).
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Death domain-containing protein CRADD (Caspase and RIP adapter with death domain) (RIP-associated protein with a death domain)
Gene names
CRADD,CRADD RAIDD
Mass
22745Da
Function
Adapter protein that associates with PIDD1 and the caspase CASP2 to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis (PubMed:9044836, PubMed:15073321, PubMed:16652156, PubMed:17159900, PubMed:17289572). Also recruits CASP2 to the TNFR-1 signaling complex through its interaction with RIPK1 and TRADD and may play a role in the tumor necrosis factor-mediated signaling pathway (PubMed:8985253).
Subellular location
Cytoplasm. Nucleus .
Tissues
Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.
Structure
Forms a complex named the PIDDosome with PIDD1 and CASP2 (PubMed:9044836, PubMed:8985253, PubMed:15073321, PubMed:16652156, PubMed:17159900, PubMed:17289572). Interacts (via Death domain) with RIPK1 (via Death domain); the interaction is direct (PubMed:9044836). Interacts with TRADD (PubMed:8985253). Interacts with TNFRSF1A (PubMed:8985253).
Involvement in disease
DISEASE: Intellectual developmental disorder, autosomal recessive 34, with variant lissencephaly (MRT34) [MIM:614499]: A disorder characterized by mild to moderate intellectual disability, megalencephaly or enlarged head circumference, and a mild variant of lissencephaly with anterior-predominant pachygyria with shallow and unusually wide sulci and mildly thickened cortex. Some patients may have seizures. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P78560
The UniProt Consortium

Data

benchmark-antibodies_anti-raidd_ct_antibody_8020_1.gif
Western blot analysis of RAIDD in MCF7 total cell lysate with RAIDD antibody at 2 µg/mL.
benchmark-antibodies_anti-raidd_ct_antibody_8020_2.jpg
Immunohistochemistry of RAIDD in human kidney tissue with RAIDD antibody at 10 µg/mL.
benchmark-antibodies_anti-raidd_ct_antibody_8020_3.gif
Immunofluorescence of RAIDD in Hela cells with RAIDD antibody at 20 µg/mL. Green: RAIDD Antibody (8020)
Blue: DAPI staining
benchmark-antibodies_anti-raidd_ct_antibody_8020_4.gif
Immunocytochemistry of RAIDD in Hela cells with RAIDD antibody at 10 µg/mL.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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