| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | PHAP III |
| available sizes | 100 µg |
rabbit anti-PHAP III polyclonal antibody 8922
$445.00
Antibody summary
- Rabbit polyclonal to PHAP III
- Suitable for: ELISA,WB,IHC-P,IF
- Isotype: IgG
- 100 µg
rabbit anti-PHAP III polyclonal antibody 8922
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Western Blot: Use at 1ug/ml. Positive control: A549 cell lysate. Immunohistochemistry: use at 2ug/ml. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. |
| Immunogen Synthetic peptide corresponding to amino acids near the carboxy terminus of human PHAP III (accession no. NP_112182) |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Acidic leucine-rich nuclear phosphoprotein 32 family member E (LANP-like protein) (LANP-L) |
| Gene names ANP32E,ANP32E |
| Protein family ANP32 family |
| Mass 30692Da |
| Function FUNCTION: Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AZ1 from the nucleosome: removes H2A.Z/H2AZ1 from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AZ1 in the nucleosome. May stabilize the evicted H2A.Z/H2AZ1-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state (PubMed:24463511). Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis. {ECO:0000269|PubMed:24463511}. |
| Subellular location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. |
| Tissues TISSUE SPECIFICITY: Expressed in peripheral blood leukocytes, colon, small intestine, prostate, thymus, spleen, skeletal muscle, liver and kidney. {ECO:0000269|PubMed:12438741}. |
| Structure SUBUNIT: Interacts with the importin alpha KPNA1 and KPNA2 (By similarity). Component of a SWR1-like complex, composed of EP400, KAT5/TIP60, TRRAP, BRD8, RUVBL1, RUVBL2, ING3 and ANP32E; the complex does not contain SRCAP. Interacts with H2A.Z/H2AZ1. {ECO:0000250, ECO:0000269|PubMed:24463511}. |
| Post-translational modification PTM: Phosphorylated. The phosphorylation is nuclear localization signal (NLS)-dependent (By similarity). {ECO:0000250}. |
| Domain DOMAIN: The H2A.Z-interacting domain (ZID) mediates a direct interaction with H2A.Z/H2AZ1. {ECO:0000269|PubMed:24463511}. |
| Target Relevance information above includes information from UniProt accession: Q9BTT0 |
| The UniProt Consortium |
Data
 |
| Western blot analysis of PHAP III expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with PHAP antibody III at 1 µg/mL. |
 |
| Immunohistochemistry of PHAP III in human small intestine tissue with PHAP III antibody at 2 µg/mL. |
 |
| Immunofluorescence of PHAP III in Human Small Intestine cells with PHAP III antibody at 10 µg/mL. |
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| # | SDS | Certificate | |
|---|---|---|---|
| Please enter your product and batch number here to retrieve product datasheet, SDS, and QC information. | |||
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