| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | PHAP (CT) |
| available sizes | 100 µg |
rabbit anti-PHAP (CT) polyclonal antibody 6885
$445.00
Antibody summary
- Rabbit polyclonal to PHAP (CT)
- Suitable for: ELISA,WB,IHC-P,IF
- Isotype: IgG
- 100 µg
rabbit anti-PHAP (CT) polyclonal antibody 6885
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Western Blot: Use at 1ug/ml. Positive control: Raji cell lysate Immunohistochemistry: use at 10ug/ml. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. |
| Immunogen Synthetic peptide corresponding to amino acids near the carboxy terminus of human PHAP (accession no. P39687). |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Homo sapiens ANP32A Acidic leucine-rich nuclear phosphoprotein 32 family member A |
| Protein names Acidic leucine-rich nuclear phosphoprotein 32 family member A |
| Alternative names Acidic nuclear phosphoprotein pp32, Leucine-rich acidic nuclear protein, Mapmodulin, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, Putative HLA-DR-associated protein I |
| Gene names ANP32A |
| Protein family Belongs to the ANP32 family |
| Function Multifunctional protein that is involved in the regulation of many processes including tumor suppression, apoptosis, cell cycle progression or transcription (PubMed:10400610, PubMed:11360199, PubMed:16341127, PubMed:18439902). Promotes apoptosis by favouring the activation of caspase-9/CASP9 and allowing apoptosome formation (PubMed:18439902). In addition, plays a role in the modulation of histone acetylation and transcription as part of the INHAT (inhibitor of histone acetyltransferases) complex. Inhibits the histone-acetyltranferase activity of EP300/CREBBP (CREB-binding protein) and EP300/CREBBP-associated factor by histone masking (PubMed:11830591). Preferentially binds to unmodified histone H3 and sterically inhibiting its acetylation and phosphorylation leading to cell growth inhibition (PubMed:16341127). Participates in other biochemical processes such as regulation of mRNA nuclear-to-cytoplasmic translocation and stability by its association with ELAVL1 (Hu-antigen R) (PubMed:18180367). Plays a role in E4F1-mediated transcriptional repression as well as inhibition of protein phosphatase 2A (PubMed:15642345, PubMed:17557114) |
| Subcellular location Nucleus, Cytoplasm, Endoplasmic reticulum |
| Structure (Microbial infection) Interacts (via C-terminus) with influenza virus C protein PB2; this interaction promotes viral replication by bridging viral replicase dimers together |
| Post-translational modification Phosphorylated on serine residues, at least in part by casein kinase 2/CK2 The N-terminus is blocked Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity) |
| Keywords 3D-structure, Cytoplasm, Direct protein sequencing, Endoplasmic reticulum, Host-virus interaction, Leucine-rich repeat, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Repeat, Repressor, Transcription, Transcription regulation |
| Sequence MEMGRRIHLELRNRTPSDVKELVLDNSRSNEGKLEGLTDEFEELEFLSTINVGLTSIANL PKLNKLKKLELSDNRVSGGLEVLAEKCPNLTHLNLSGNKIKDLSTIEPLKKLENLKSLDL FNCEVTNLNDYRENVFKLLPQLTYLDGYDRDDKEAPDSDAEGYVEGLDDEEEDEDEEEYD EDAQVVEDEEDEDEEEEGEEEDVSGEEEEDEEGYNDGEVDDEEDEEELGEEERGQKRKRE PEDEGEDDD |
| UniProt accession: P39687 |
Data
 |
| Western blot analysis of PHAP expression in human Raji cell llysate with PHAP antibody at 1 µg/mL. |
 |
| Immunohistochemistry of PHAP in human small intestine tissue with PHAP antibody at 10 µg/mL. |
 |
| Immunofluorescence of PHAP in human small intestine tissue with PHAP antibody at 20 µg/mL. |
FAQ & Publications
Frequently Asked Questions
What are the recommended applications and dilutions for the rabbit anti-PHAP (CT) polyclonal antibody 6885?
This antibody is suitable for Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF), and ELISA. Recommended dilutions are 1 µg/mL for Western Blot and 10 µg/mL for Immunohistochemistry. Users should optimize concentrations for their specific applications.
How should the rabbit anti-PHAP (CT) polyclonal antibody 6885 be stored to maintain stability?
The antibody should be stored at -20°C and is stable for at least one year under these conditions. It is important to avoid multiple freeze-thaw cycles to preserve antibody integrity.
What is the host species and clonality of the anti-PHAP (CT) antibody 6885?
The antibody is a rabbit polyclonal antibody with an IgG isotype.
What is the immunogen used to generate the rabbit anti-PHAP (CT) polyclonal antibody 6885?
The immunogen is a synthetic peptide corresponding to amino acids near the carboxy terminus of the human PHAP protein (accession no. P39687).
Which secondary antibodies are compatible with the rabbit anti-PHAP (CT) polyclonal antibody 6885?
Compatible secondary antibodies include goat anti-rabbit IgG, H&L chain specific antibodies conjugated with peroxidase, biotin, or FITC, including cross-adsorbed variants.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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