rabbit anti-p70S6K Kinase polyclonal antibody 8413

$522.00

Antibody summary

Rabbit polyclonal to p70S6K Kinase
Suitable for: WB
Isotype: Whole IgG
100 µg

SKU: 8413parent Category: antibody Tags: p70S6K Kinase, polyclonal, rabbit

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	p70S6K Kinase
available sizes	100 µg

rabbit anti-p70S6K Kinase polyclonal antibody 8413

antibody

Tested applications WB
Recommended dilutions Western Blot: 0.05-0.4 ug/ml.
Immunogen Synthetic peptide representing a portion of the C-terminus of the protein (LocusLink ID 6197).
Size and concentration 100µg and lot specific
Form liquid
Storage Instructions Store at 2 - 8°C. Antibody is stable at 2 - 8°C for 1 year.
Storage buffer Tris-citrate/phosphate buffer, pH 7 to 8 contai
Purity immunogen affinty purifcation
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Ribosomal protein S6 kinase beta-1 (S6K-beta-1) (S6K1) (EC 2.7.11.1) (70 kDa ribosomal protein S6 kinase 1) (P70S6K1) (p70-S6K 1) (Ribosomal protein S6 kinase I) (Serine/threonine-protein kinase 14A) (p70 ribosomal S6 kinase alpha) (p70 S6 kinase alpha) (p70 S6K-alpha) (p70 S6KA)
Gene names RPS6KB1,RPS6KB1 STK14A
Protein family Protein kinase superfamily, AGC Ser/Thr protein kinase family, S6 kinase subfamily
Mass 59140Da
Function FUNCTION: Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression (PubMed:11500364, PubMed:12801526, PubMed:14673156, PubMed:15071500, PubMed:15341740, PubMed:16286006, PubMed:17052453, PubMed:17053147, PubMed:17936702, PubMed:18952604, PubMed:19085255, PubMed:19720745, PubMed:19935711, PubMed:19995915, PubMed:23429703, PubMed:28178239, PubMed:22017876). Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD (PubMed:11500364, PubMed:12801526, PubMed:14673156, PubMed:15071500, PubMed:15341740, PubMed:16286006, PubMed:17052453, PubMed:17053147, PubMed:17936702, PubMed:18952604, PubMed:19085255, PubMed:19720745, PubMed:19935711, PubMed:19995915, PubMed:23429703, PubMed:28178239, PubMed:22017876). Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex (PubMed:16286006). Upon mitogenic stimulation, phosphorylation by the mechanistic target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation (PubMed:16286006). The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B (PubMed:16286006). Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis (PubMed:17053147). Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR (PubMed:15341740). In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2 (PubMed:11500364). Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling (PubMed:19720745, PubMed:19935711, PubMed:19995915). Also involved in feedback regulation of mTORC1 and mTORC2 by phosphorylating DEPTOR (PubMed:22017876). Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function (By similarity). Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex (PubMed:17936702). The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function (PubMed:17936702). Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1 (PubMed:18952604). In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B (PubMed:17052453). May be involved in cytoskeletal rearrangement through binding to neurabin (By similarity). Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR (PubMed:23429703). Following activation by mTORC1, phosphorylates EPRS and thereby plays a key role in fatty acid uptake by adipocytes and also most probably in interferon-gamma-induced translation inhibition (PubMed:28178239). {ECO:0000250\|UniProtKB:P67999, ECO:0000250\|UniProtKB:Q8BSK8, ECO:0000269\|PubMed:11500364, ECO:0000269\|PubMed:12801526, ECO:0000269\|PubMed:14673156, ECO:0000269\|PubMed:15071500, ECO:0000269\|PubMed:15341740, ECO:0000269\|PubMed:16286006, ECO:0000269\|PubMed:17052453, ECO:0000269\|PubMed:17053147, ECO:0000269\|PubMed:17936702, ECO:0000269\|PubMed:18952604, ECO:0000269\|PubMed:19085255, ECO:0000269\|PubMed:19720745, ECO:0000269\|PubMed:19935711, ECO:0000269\|PubMed:19995915, ECO:0000269\|PubMed:22017876, ECO:0000269\|PubMed:23429703, ECO:0000269\|PubMed:28178239}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1; Evidence={ECO:0000269\|PubMed:17936702, ECO:0000269\|PubMed:18952604, ECO:0000269\|PubMed:19085255, ECO:0000269\|PubMed:28178239}; CATALYTIC ACTIVITY: Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1; Evidence={ECO:0000269\|PubMed:17936702, ECO:0000269\|PubMed:18952604, ECO:0000269\|PubMed:19085255, ECO:0000269\|PubMed:28178239};
Subellular location SUBCELLULAR LOCATION: Synapse, synaptosome {ECO:0000250}. Mitochondrion outer membrane. Mitochondrion. Note=Colocalizes with URI1 at mitochondrion.; SUBCELLULAR LOCATION: [Isoform Alpha I]: Nucleus. Cytoplasm.; SUBCELLULAR LOCATION: [Isoform Alpha II]: Cytoplasm.
Tissues TISSUE SPECIFICITY: Widely expressed. {ECO:0000269\|PubMed:9804755}.
Structure SUBUNIT: Interacts with PPP1R9A/neurabin-1 (By similarity). Interacts with RPTOR (PubMed:12150926). Interacts with IRS1 (PubMed:18952604). Interacts with EIF3B and EIF3C (PubMed:16286006). Interacts with TRAF4 (PubMed:12801526). Interacts with POLDIP3 (PubMed:15341740). Interacts (via N-terminus) with IER5 (PubMed:26496226). {ECO:0000250\|UniProtKB:P67999, ECO:0000269\|PubMed:12150926, ECO:0000269\|PubMed:12801526, ECO:0000269\|PubMed:15341740, ECO:0000269\|PubMed:16286006, ECO:0000269\|PubMed:18952604, ECO:0000269\|PubMed:26496226}.
Post-translational modification PTM: Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (PubMed:29236692, PubMed:18925875, PubMed:19085255, PubMed:22017876, PubMed:23429703). Activated by phosphorylation at Thr-252 by PDPK1 (PubMed:9445476, PubMed:19864428). Dephosphorylation by PPP1CC at Thr-412 in mitochondrion (PubMed:17936702). {ECO:0000269\|PubMed:17936702, ECO:0000269\|PubMed:18925875, ECO:0000269\|PubMed:19085255, ECO:0000269\|PubMed:19864428, ECO:0000269\|PubMed:22017876, ECO:0000269\|PubMed:23429703, ECO:0000269\|PubMed:29236692, ECO:0000269\|PubMed:9445476}.
Target Relevance information above includes information from UniProt accession : P23443
The UniProt Consortium

Data

benchmark-antibodies_anti-p70s6k_kinase_antibody_8413_1.jpg

Detection of human and mouse p70S6K by western blot. Samples: Whole cell lysate (50 µg ) from HeLa and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p70S6K antibody 8413 (lot 8413-2) used for WB at 0.05 µg /ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.