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rabbit anti-p53R2 (NT) polyclonal antibody 7406

$445.00

Antibody summary

  • Rabbit polyclonal to p53R2 (NT)
  • Suitable for: ELISA,WB,IHC-P,IF
  • Isotype: Whole IgG
  • 100 µg
SKU: 7406parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

p53R2 (NT)

available sizes

100 µg

Available product – rabbit anti-p53R2 (NT) polyclonal antibody 7406

antibody
Tested applications
WB,IHC,IHC,ICC/IF,ELISA
Recommended dilutions
Immunoblotting: use at 1ug/mL. A band of ~39kDa is detected

IHC: use at 1ug/mL.

Immunofluorescence: use at 20ugml.
Immunogen
Peptide corresponding to aa 2-17 of human p53R2.
Size and concentration
100µg and 1 mg/mL
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4, 0.02% NaN3.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Ribonucleoside-diphosphate reductase subunit M2 B (EC 1.17.4.1) (TP53-inducible ribonucleotide reductase M2 B) (p53-inducible ribonucleotide reductase small subunit 2-like protein) (p53R2)
Gene names
RRM2B,RRM2B P53R2
Protein family
Ribonucleoside diphosphate reductase small chain family
Mass
40737Da
Function
FUNCTION: Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. {ECO:0000269|PubMed:10716435, ECO:0000269|PubMed:11517226, ECO:0000269|PubMed:11719458}.
Catalytic activity
CATALYTIC ACTIVITY: Reaction=[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate; Xref=Rhea:RHEA:23252, Rhea:RHEA-COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:29950, ChEBI:CHEBI:50058, ChEBI:CHEBI:57930, ChEBI:CHEBI:73316; EC=1.17.4.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10014, ECO:0000269|PubMed:16376858};
Subellular location
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocates from cytoplasm to nucleus in response to DNA damage.
Tissues
TISSUE SPECIFICITY: Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma. {ECO:0000269|PubMed:14583450}.
Structure
SUBUNIT: Heterotetramer with large (RRM1) subunit. Interacts with p53/TP53. Interacts with RRM1 in response to DNA damage. {ECO:0000269|PubMed:11517226, ECO:0000269|PubMed:12615712, ECO:0000269|PubMed:16376858, ECO:0000269|PubMed:19728742, ECO:0000269|Ref.17}.
Target Relevance information above includes information from UniProt accession : Q7LG56
The UniProt Consortium

Data

benchmark-antibodies_anti-p53r2_nt_antibody_7406_1.gif
Western blot analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.
benchmark-antibodies_anti-p53r2_nt_antibody_7406_2.jpg
Immunohistochemistry of p53R2 in human lung tissue with p53R2 antibody at 1 µg/mL.
benchmark-antibodies_anti-p53r2_nt_antibody_7406_3.jpg
Immunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 µg/mL.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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