| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | NIK (CT) |
| available sizes | 100 µg |
rabbit anti-NIK (CT) polyclonal antibody 3610
$478.00
Antibody summary
- Rabbit polyclonal to NIK (CT)
- Suitable for: ELISA,WB,IF,ICC
- Isotype: IgG
- 100 µg
rabbit anti-NIK (CT) polyclonal antibody 3610
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: Recommended concentration: 2-4ug/ml. Optimal dilution should be determined by End user. Positive control: Whole cell lysate of NIK-transfected 293 cells. |
| Immunogen Synthetic peptide corresponding to aa 931-947 of human NIK (accession no. Q99558). |
| Size and concentration 100µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for 3 months at 4°C and at least 1 year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, 1mg/ml, pH 7.4, 0.02% NaN3. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Mitogen-activated protein kinase kinase kinase 14 (EC 2.7.11.25) (NF-kappa-beta-inducing kinase) (HsNIK) (Serine/threonine-protein kinase NIK) |
| Gene names MAP3K14,MAP3K14 NIK |
| Protein family Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase kinase subfamily |
| Mass 104042Da |
| Function FUNCTION: Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Phosphorylates CHUK/IKKA, thereby promoting proteolytic processing of NFKB2/P100, which leads to NF-kappa-B activation via the non-canonical pathway (PubMed:25406581, PubMed:29230214). Has an essential role in the non-canonical NF-kappa-B signaling that regulates genes encoding molecules involved in B-cell survival, lymphoid organogenesis, and immune response (PubMed:25406581). Could act in a receptor-selective manner. {ECO:0000269|PubMed:15084608, ECO:0000269|PubMed:25406581}. |
| Catalytic activity CATALYTIC ACTIVITY: Reaction=L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+); Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.25; CATALYTIC ACTIVITY: Reaction=L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+); Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.25; |
| Subellular location SUBCELLULAR LOCATION: Cytoplasm. |
| Tissues TISSUE SPECIFICITY: Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon. {ECO:0000269|PubMed:9020361}. |
| Structure SUBUNIT: Interacts with TRAF2, TRAF5, TRAF6, IKKA and NFKB2/P100 (By similarity). Interacts with TRAF3 and PELI3. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2. Interacts with GRB10. Interacts with ZFP91. Interacts with NLRP12; this interaction promotes proteasomal degradation of MAP3K14. Directly interacts with DDX3X (PubMed:30341167). Interacts (via C-terminus and kinase domain) with PPPC3A (via N-terminus) and PPP3CB (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:Q9WUL6, ECO:0000269|PubMed:12853971, ECO:0000269|PubMed:12874243, ECO:0000269|PubMed:15084608, ECO:0000269|PubMed:15173580, ECO:0000269|PubMed:15951441, ECO:0000269|PubMed:17237370, ECO:0000269|PubMed:20682767, ECO:0000269|PubMed:30341167}. |
| Post-translational modification PTM: Autophosphorylated. Phosphorylation at Thr-559 is required to activate its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization. {ECO:0000269|PubMed:20501937, ECO:0000269|PubMed:20682767}.; PTM: Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it. |
| Involvement in disease DISEASE: Immunodeficiency 112 (IMD112) [MIM:620449]: An autosomal recessive, primary immunologic disorder characterized by variable abnormalities affecting lymphoid immunity, including hypogammaglobulinemia, lymphopenia or paradoxical lymphocytosis, and recurrent bacterial, viral, and fungal infections. {ECO:0000269|PubMed:25406581, ECO:0000269|PubMed:29230214}. Note=The disease is caused by variants affecting the gene represented in this entry. |
| Target Relevance information above includes information from UniProt accession: Q99558 |
| The UniProt Consortium |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What applications is the rabbit anti-NIK (CT) polyclonal antibody 3610 validated for?
This antibody has been tested and is suitable for ELISA, Western blot (WB), immunofluorescence (IF), and immunocytochemistry (ICC).
How should the rabbit anti-NIK (CT) polyclonal antibody be stored to maintain stability?
The antibody is stable for 3 months when stored at 4°C and for at least 1 year at -20°C. It is recommended to avoid multiple freeze-thaw cycles to preserve antibody integrity.
What is the immunogen used to generate this rabbit anti-NIK (CT) antibody?
The immunogen is a synthetic peptide corresponding to amino acids 931-947 of human NIK (accession no. Q99558).
What is the antibody concentration and recommended dilution for immunoblotting applications?
The antibody concentration is 1 mg/mL. For immunoblotting, a recommended working concentration is 2-4 µg/mL, though the optimal dilution should be determined by the end user.
Which secondary antibodies are compatible with the rabbit anti-NIK (CT) polyclonal antibody?
Compatible secondary antibodies include goat anti-rabbit IgG, H&L chain specific polyclonal antibodies conjugated with peroxidase, biotin, or FITC, available in both standard and cross-absorbed forms.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
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