| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | MyoD (Phospho-Ser200) |
| available sizes | 100 µL |
rabbit anti-MyoD (Phospho-Ser200) polyclonal antibody 6932
$366.00
Antibody summary
- Rabbit polyclonal to MyoD (Phospho-Ser200)
- Suitable for: WB
- Isotype: Whole IgG
- 100 µl
rabbit anti-MyoD (Phospho-Ser200) polyclonal antibody 6932
| antibody |
|---|
| Tested applications WB |
| Recommended dilutions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~40kDa is detected. These are recommended working dilutions. End users should determine optimal dilutions for their applications. |
| Immunogen Peptide sequence that includes phosphorylation site of Serine 200 (A-S-S(p)-P-R) derived from human MyoD and conjugated to KLH. |
| Size and concentration 100µL and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. |
| Storage buffer PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Myoblast determination protein 1 (Class C basic helix-loop-helix protein 1) (bHLHc1) (Myogenic factor 3) (Myf-3) |
| Mass 34501Da |
| Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity). {ECO:0000250}. |
| Subellular location Nucleus. |
| Structure Efficient DNA binding requires dimerization with another bHLH protein. Seems to form active heterodimers with ITF-2. Interacts with SUV39H1. Interacts with DDX5. Interacts with CHD2. Interacts with TSC22D3 (By similarity). Interacts with SETD3 (By similarity). Interacts with P-TEFB complex; promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation (By similarity) (PubMed:12037670). Interacts with CSRP3. Interacts with NUPR1 (By similarity). {ECO:0000250|UniProtKB:P10085, ECO:0000269|PubMed:12037670, ECO:0000269|PubMed:16858404, ECO:0000269|PubMed:24860983}. |
| Post-translational modification Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation. {ECO:0000269|PubMed:12037670}.; Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity). {ECO:0000250}.; Ubiquitinated on the N-terminus; which is required for proteasomal degradation. {ECO:0000269|PubMed:9774340}.; Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity. {ECO:0000269|PubMed:22215600}. |
| Involvement in disease DISEASE: Congenital myopathy 17 (CMYP17) [MIM:618975]: An autosomal recessive muscular disorder characterized by hypotonia and respiratory insufficiency apparent soon after birth, high diaphragmatic dome on imaging, poor overall growth, pectus excavatum, dysmorphic facies, and renal anomalies in some affected individuals. Additional variable features include delayed motor development, mildly decreased endurance, distal arthrogryposis, and lung hypoplasia resulting in early death. {ECO:0000269|PubMed:26733463, ECO:0000269|PubMed:30403323, ECO:0000269|PubMed:31260566}. Note=The disease is caused by variants affecting the gene represented in this entry. |
| Target Relevance information above includes information from UniProt accession: P15172 |
| The UniProt Consortium |
Data
| No results found |
Publications
Publications
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| We haven't added any publications to our database yet. | |||
Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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