Weight | 1 lbs |
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Dimensions | 9 × 5 × 2 in |
host | rabbit |
isotype | IgG |
clonality | polyclonal |
concentration | 1 mg/mL |
applications | ICC/IF, WB |
reactivity | Smac (mouse CT) |
available sizes | 100 µg |
rabbit anti-mSmac (CT) polyclonal antibody 8528
$445.00
Antibody summary
- Rabbit polyclonal to mSmac (CT)
- Suitable for: ELISA,WB,IHC-P,IF,IP
- Isotype: IgG
- 100 µg
rabbit anti-mSmac (CT) polyclonal antibody 8528
antibody |
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Tested applications WB,IHC,IHC,ICC/IF,ELISA |
Recommended dilutions Immunoblotting: use at 1ug/mL. Positive control: Mouse heart tissue lysate. Immunohistochemistry: use at 2ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. |
Immunogen Synthetic peptide corresponding to aa 222-237 of mouse Smac (accession no. AF203914). |
Size and concentration 100µg and lot specific |
Form liquid |
Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
Storage buffer PBS, pH 7.4. |
Purity peptide affinty purifcation |
Clonality polyclonal |
Isotype IgG |
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
Isotype control Rabbit polyclonal - Isotype Control |
target relevance |
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Protein names Diablo IAP-binding mitochondrial protein (Diablo homolog, mitochondrial) (Direct IAP-binding protein with low pI) (Second mitochondria-derived activator of caspase) (Smac) [Cleaved into: Diablo IAP-binding mitochondrial protein, cleaved form] |
Gene names Diablo,Diablo Smac |
Mass 26820Da |
Function Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases (By similarity). |
Subellular location Mitochondrion. Cytoplasm, cytosol. Note=Released into the cytosol in a PARL-dependent manner when cells undergo apoptosis. |
Tissues Highest expression found in heart, liver, kidney and testis. |
Structure Homodimer (By similarity). Interacts with BIRC2/c-IAP1 (By similarity). Interacts with BIRC6/bruce (By similarity). Interacts with BIRC7/livin (By similarity). Interacts with the monomeric and dimeric form of BIRC5/survivin (By similarity). Interacts with XIAP/BIRC4 (via BIR3 domain) (By similarity). Interacts with BEX3 (PubMed:15178455). Interacts with AREL1 (via HECT domain); in the cytoplasm following induction of apoptosis (PubMed:23479728). |
Post-translational modification Ubiquitinated by BIRC7/livin.; The precursor form is proteolytically cleaved by mitochondrial processing peptidase MPP to remove the transit peptide and produce an intermediate form. This is then processed by PARL to produce the mature cleaved form which is released from mitochondria into the cytosol in apoptotic cells. |
Target Relevance information above includes information from UniProt accession: Q9JIQ3 |
The UniProt Consortium |
Publications
pmid | title | authors | citation |
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We haven't added any publications to our database yet. |
relevant to this product |
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Western blot IHC ICC |
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