| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | MMP 9 |
| available sizes | 100 µg |
rabbit anti-MMP9 polyclonal antibody 6573
$376.00
Antibody summary
- Rabbit polyclonal to MMP9
- Suitable for: WB
- Isotype: Whole IgG
- 100 µg
rabbit anti-MMP9 polyclonal antibody 6573
| antibody |
|---|
| Tested applications WB |
| Recommended dilutions Immunoblotting: use at 1:500-1:1,000 dilution. A band at 92 kD is detected. |
| Immunogen Synthetic peptide corresponding to aa 540-552 of human MMP 9. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C to -70°C. Store antibody in appropriate aliquots to avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity immunogen affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Homo sapiens MMP9 Matrix metalloproteinase-9 |
| Protein names Matrix metalloproteinase-9 |
| Alternative names 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B |
| Gene names MMP9 |
| Protein family Belongs to the peptidase M10A family |
| Function Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (PubMed:12879005, PubMed:1480034, PubMed:2551898). Could play a role in bone osteoclastic resorption (By similarity). Cleaves KiSS1 at a Gly-|-Leu bond (PubMed:12879005). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed:32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (PubMed:1480034). Degrades fibronectin but not laminin or Pz-peptide |
| Catalytic activity Cleavage of gelatin types I and V and collagen types IV and V. |
| Subcellular location Secreted, extracellular space, extracellular matrix |
| Structure (Microbial infection) Interacts with Staphylococcus aureus protein SSL5; this interaction inhibits MMP9 activity |
| Post-translational modification Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9 N- and O-glycosylated |
| Involvement in disease Intervertebral disc disease A common musculo-skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Metaphyseal anadysplasia 2 A bone development disorder characterized by skeletal anomalies that resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. |
| Keywords 3D-structure, Calcium, Collagen degradation, Direct protein sequencing, Disulfide bond, Extracellular matrix, Glycoprotein, Hydrolase, Metal-binding, Metalloprotease, Protease, Proteomics identification, Reference proteome, Repeat, Secreted, Signal, Zinc, Zymogen |
| Sequence MSLWQPLVLVLLVLGCCFAAPRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYTRVAEM RGESKSLGPALLLLQKQLSLPETGELDSATLKAMRTPRCGVPDLGRFQTFEGDLKWHHHN ITYWIQNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRVYSRDADIVIQFGVAEHGDGYP FDGKDGLLAHAFPPGPGIQGDAHFDDDELWSLGKGVVVPTRFGNADGAACHFPFIFEGRS YSACTTDGRSDGLPWCSTTANYDTDDRFGFCPSERLYTQDGNADGKPCQFPFIFQGQSYS ACTTDGRSDGYRWCATTANYDRDKLFGFCPTRADSTVMGGNSAGELCVFPFTFLGKEYST CTSEGRGDGRLWCATTSNFDSDKKWGFCPDQGYSLFLVAAHEFGHALGLDHSSVPEALMY PMYRFTEGPPLHKDDVNGIRHLYGPRPEPEPRPPTTTTPQPTAPPTVCPTGPPTVHPSER PTAGPTGPPSAGPTGPPTAGPSTATTVPLSPVDDACNVNIFDAIAEIGNQLYLFKDGKYW RFSEGRGSRPQGPFLIADKWPALPRKLDSVFEERLSKKLFFFSGRQVWVYTGASVLGPRR LDKLGLGADVAQVTGALRSGRGKMLLFSGRRLWRFDVKAQMVDPRSASEVDRMFPGVPLD THDVFQYREKAYFCQDRFYWRVSSRSELNQVDQVGYVTYDILQCPED |
| UniProt accession: P14780 |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What applications has the rabbit anti-MMP9 polyclonal antibody 6573 been validated for?
This antibody has been tested and is recommended for use in Western blotting (WB) and immunocytochemistry/immunofluorescence (ICC/IF) applications.
How should the rabbit anti-MMP9 polyclonal antibody 6573 be stored to maintain stability?
The antibody should be stored at temperatures between -20°C and -70°C and aliquoted appropriately to avoid repeated freeze-thaw cycles. Under these conditions, it remains stable for at least one year.
What is the immunogen used to raise the rabbit anti-MMP9 polyclonal antibody 6573?
The immunogen is a synthetic peptide corresponding to amino acids 540 to 552 of human MMP9.
Which secondary antibodies are compatible with the rabbit anti-MMP9 polyclonal antibody 6573 for detection?
Compatible secondary antibodies include goat anti-rabbit IgG, H&L chain specific, available conjugated with peroxidase, biotin, or FITC, including cross-absorbed versions for reduced background.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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