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rabbit anti-MCT-1 polyclonal antibody 6529

$376.00

Antibody summary

  • Rabbit polyclonal to MCT-1
  • Suitable for: WB
  • Isotype: Whole IgG
  • 100 µg
SKU: 6529parent Category: Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

MCT-1

available sizes

100 µg

rabbit anti-MCT-1 polyclonal antibody 6529

antibody
Tested applications
WB
Recommended dilutions
Immunoblotting: use at 1:500-1:1,000 dilution. A band at approx. 20 kD is detected.
Immunogen
Peptide corresponding to aa 3-14 of human MCT-1 protein.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Monocarboxylate transporter 1 (MCT 1) (Solute carrier family 16 member 1)
Gene names
SLC16A1,SLC16A1 MCT1
Protein family
Major facilitator superfamily, Monocarboxylate porter (TC 2.A.1.13) family
Mass
53944Da
Function
Bidirectional proton-coupled monocarboxylate transporter (PubMed:12946269, PubMed:33333023, PubMed:32946811). Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, acetate and the ketone bodies acetoacetate and beta-hydroxybutyrate, and thus contributes to the maintenance of intracellular pH (PubMed:12946269, PubMed:33333023). The transport direction is determined by the proton motive force and the concentration gradient of the substrate monocarboxylate. MCT1 is a major lactate exporter (By similarity). Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis (By similarity). Facilitates the protonated monocarboxylate form of succinate export, that its transient protonation upon muscle cell acidification in exercising muscle and ischemic heart (PubMed:32946811). Functions via alternate outward- and inward-open conformation states. Protonation and deprotonation of 309-Asp is essential for the conformational transition (PubMed:33333023).
Catalytic activity
CATALYTIC ACTIVITY: Reaction=(S)-lactate(in) + H(+)(in) = (S)-lactate(out) + H(+)(out); Xref=Rhea:RHEA:29415, ChEBI:CHEBI:15378, ChEBI:CHEBI:16651; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29416; Evidence=; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:29417; Evidence=; CATALYTIC ACTIVITY: Reaction=acetate(out) + H(+)(out) = acetate(in) + H(+)(in); Xref=Rhea:RHEA:71803, ChEBI:CHEBI:15378, ChEBI:CHEBI:30089; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71804; Evidence=; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:71805; Evidence=; CATALYTIC ACTIVITY: Reaction=acetoacetate(out) + H(+)(out) = acetoacetate(in) + H(+)(in); Xref=Rhea:RHEA:71775, ChEBI:CHEBI:13705, ChEBI:CHEBI:15378; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71776; Evidence=; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:71777; Evidence=; CATALYTIC ACTIVITY: Reaction=H(+)(out) + pyruvate(out) = H(+)(in) + pyruvate(in); Xref=Rhea:RHEA:64720, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378; Evidence=; CATALYTIC ACTIVITY: Reaction=(R)-3-hydroxybutanoate(out) + H(+)(out) = (R)-3-hydroxybutanoate(in) + H(+)(in); Xref=Rhea:RHEA:71795, ChEBI:CHEBI:10983, ChEBI:CHEBI:15378; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71796; Evidence=; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:71797; Evidence=; CATALYTIC ACTIVITY: Reaction=3-methyl-2-oxobutanoate(out) + H(+)(out) = 3-methyl-2-oxobutanoate(in) + H(+)(in); Xref=Rhea:RHEA:71783, ChEBI:CHEBI:11851, ChEBI:CHEBI:15378; Evidence=; CATALYTIC ACTIVITY: Reaction=4-methyl-2-oxopentanoate(out) + H(+)(out) = 4-methyl-2-oxopentanoate(in) + H(+)(in); Xref=Rhea:RHEA:71779, ChEBI:CHEBI:15378, ChEBI:CHEBI:17865; Evidence=; CATALYTIC ACTIVITY: Reaction=2 H(+)(in) + succinate(in) = 2 H(+)(out) + succinate(out); Xref=Rhea:RHEA:29303, ChEBI:CHEBI:15378, ChEBI:CHEBI:30031; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29304; Evidence=;
Subellular location
Cell membrane ; Multi-pass membrane protein. Basolateral cell membrane ; Multi-pass membrane protein. Apical cell membrane ; Multi-pass membrane protein. Note=Expression at the cell surface requires the ancillary proteins BSG and EMB. Binds preferentially to BSG.
Tissues
Widely expressed (PubMed:15901598, PubMed:15505343, PubMed:12115955). Detected in heart and in blood lymphocytes and monocytes (at protein level) (PubMed:15505343).
Structure
Interacts with EMB; interaction mediates SLC16A1 targeting to the plasma membrane (By similarity). Interacts with isoform 2 of BSG; interaction mediates SLC16A1 targeting to the plasma membrane (PubMed:17127621, PubMed:25957687, PubMed:33333023).
Domain
TOPO_DOM 1
Involvement in disease
DISEASE: Symptomatic deficiency in lactate transport (SDLT) [MIM:245340]: Deficiency of lactate transporter may result in an acidic intracellular environment created by muscle activity with consequent degeneration of muscle and release of myoglobin and creatine kinase. This defect might compromise extreme performance in otherwise healthy individuals. Note=The disease is caused by variants affecting the gene represented in this entry.; DISEASE: Hyperinsulinemic hypoglycemia, familial, 7 (HHF7) [MIM:610021]: A form of hyperinsulinemic hypoglycemia, a clinically and genetically heterogeneous disorder characterized by inappropriate insulin secretion from the pancreatic beta-cells in the presence of low blood glucose levels. HHF7 features include exercise-induced hyperinsulinism, loss of consciousness due to hypoglycemia, and hypoglycemic seizures. HHF7 inheritance is autosomal dominant. Note=The disease is caused by variants affecting the gene represented in this entry.; DISEASE: Monocarboxylate transporter 1 deficiency (MCT1D) [MIM:616095]: A metabolic disorder characterized by recurrent ketoacidosis, a pathologic state due to ketone formation exceeding ketone utilization. The clinical consequences of ketoacidosis are vomiting, osmotic diuresis, dehydration, and Kussmaul breathing. The condition may progress to decreased consciousness and, ultimately, death. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P53985
The UniProt Consortium

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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Western blot
IHC
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Batch Information
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