| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | JunD (Phospho-Ser255) |
| available sizes | 100 µL |
rabbit anti-JunD (Phospho-Ser255) polyclonal antibody 1360
$366.00
Antibody summary
- Rabbit polyclonal to JunD (Phospho-Ser255)
- Suitable for: WB,IHC
- Isotype: Whole IgG
- 100 µl
rabbit anti-JunD (Phospho-Ser255) polyclonal antibody 1360
| antibody |
|---|
| Tested applications WB,IHC,IHC |
| Recommended dilutions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~38kDa is detected. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications. |
| Immunogen Peptide sequence that includes phosphorylation site of serine 255 (G-E-S(p)-P-P) derived from human JunD and conjugated to KLH. |
| Size and concentration 100µL and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. |
| Storage buffer PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Transcription factor JunD (Transcription factor AP-1 subunit JunD) |
| Gene names JUND,JUND |
| Protein family BZIP family, Jun subfamily |
| Mass 35174Da |
| Function FUNCTION: Transcription factor binding AP-1 sites (PubMed:9989505). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGA[GC]TCA-5' and enhancing their transcriptional activity (PubMed:28981703, PubMed:9989505). {ECO:0000269|PubMed:28981703, ECO:0000269|PubMed:9989505}. |
| Subellular location SUBCELLULAR LOCATION: Nucleus. |
| Structure SUBUNIT: Heterodimer; binds DNA as a heterodimer (PubMed:28981703). Component of an AP-1 transcription factor complex composed of JUN-FOS heterodimers (By similarity). As part of the AP-1 transcription factor complex, forms heterodimers with FOS proteins, thereby binding to the AP-1 consensus sequence and stimulating transcription (By similarity). Forms heterodimers with FOSB; thereby binding to the AP-1 consensus sequence (PubMed:28981703). Interacts (via MBM motif) with MEN1; this interaction represses transcriptional activation (PubMed:22327296, PubMed:9989505). Interacts with MAPK10; this interaction is inhibited in the presence of MEN1 (PubMed:22327296). {ECO:0000250|UniProtKB:P13346, ECO:0000269|PubMed:22327296, ECO:0000269|PubMed:28981703, ECO:0000269|PubMed:9989505}. |
| Post-translational modification PTM: Phosphorylated by MAP kinases MAPK8 and MAPK10; phosphorylation is inhibited in the presence of MEN1. {ECO:0000269|PubMed:22327296}. |
| Domain DOMAIN: Binds DNA via bZIP domain; DNA-binding is under control of cellular redox homeostasis (in vitro) (PubMed:28981703). To enable DNA binding, the bZIP domain must undergo a conformational rearrangement which requires the reduction of the interchain disulfide bond between FosB and JunD (in vitro) (PubMed:28981703). {ECO:0000269|PubMed:28981703}. |
| Target Relevance information above includes information from UniProt accession: P17535 |
| The UniProt Consortium |
Data
| No results found |
Publications
Publications
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|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Protocols
| relevant to this product |
|---|
| Western blot IHC |
Documents
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