rabbit anti-HO1 polyclonal antibody 1028

$100.00 – $2,600.00

Antibody summary

Rabbit polyclonal to HO-1 (Heme oxygenase-1)
Suitable for: IP
Reacts with: Hu
Isotype: IgG
100 µL, 10 µl

available sizes

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SKU: 1028parent Categories: antibody, oxidative stress Tag: HO-1

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	IP
reactivity	human
available sizes	10 µL, 100 µL

rabbit anti- ho1 polyclonal antibody 1028

antibody
Database link: human P09601
Tested applications IP
Recommended dilutions Immunoprecipitation (IP) 6 µg/mg lysate
Immunogen Between 1 and 50
Size and concentration 100µL and 1 mg/mL
Form liquid
Storage Instructions Store at 2-8°C. Expires 1 year from date of receipt.
Storage buffer Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Heme oxygenase 1 (HO-1) (EC 1.14.14.18) [Cleaved into: Heme oxygenase 1 soluble form]
Gene names HMOX1,HMOX1 HO HO1
Protein family Heme oxygenase family
Mass 32819Da
Function [Heme oxygenase 1]: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:11121422, PubMed:19556236, PubMed:7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707).; [Heme oxygenase 1]: (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.; [Heme oxygenase 1 soluble form]: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.
Catalytic activity CATALYTIC ACTIVITY: Reaction=heme b + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = biliverdin IXalpha + CO + Fe(2+) + H(+) + 3 H2O + 3 oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:21764, Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17245, ChEBI:CHEBI:29033, ChEBI:CHEBI:57618, ChEBI:CHEBI:57991, ChEBI:CHEBI:58210, ChEBI:CHEBI:60344; EC=1.14.14.18; Evidence=; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:21765; Evidence=;
Subellular location Endoplasmic reticulum membrane ; Single-pass type IV membrane protein ; Cytoplasmic side .
Tissues Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).
Structure (Microbial infection) Interacts with SARS-CoV-2 ORF3A protein; the interaction promotes ORF3A-induced autophagy but is unlikely to be involved in ORF3A-mediated induction of reticulophagy.; Homodimer and higher order homooligomer (PubMed:19556236). Oligomerization is crucial for its stability and function in the endoplasmic reticulum (PubMed:19556236). Interacts with FLVCR2; this interaction is potentiated in the presence of heme.
Post-translational modification A soluble form arises by proteolytic removal of the membrane anchor.
Domain Th
Involvement in disease DISEASE: Heme oxygenase 1 deficiency (HMOX1D) [MIM:614034]: A disease characterized by impaired stress hematopoiesis, resulting in marked erythrocyte fragmentation and intravascular hemolysis, coagulation abnormalities, endothelial damage, and iron deposition in renal and hepatic tissues. Clinical features include persistent hemolytic anemia, asplenia, nephritis, generalized erythematous rash, growth retardation and hepatomegaly. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P09601
The UniProt Consortium

Data

WB-image-anti-HO-1 (Heme oxygenase-1) antibody-1028_1

Detection of human HO-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HO-1 antibody #1028 used for IP at 6 µg/mg lysate. HO-1 was also immunoprecipitated by rabbit anti-HO-1 antibody, which recognizes a downstream epitope. For blotting immunoprecipitated HO-1 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Publications

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.