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rabbit anti-HNF4 α (phospho-Ser304) polyclonal antibody 8472

$366.00

Antibody summary

  • Rabbit polyclonal to HNF4 α (phospho-Ser304)
  • Suitable for: WB
  • Isotype: Whole IgG
  • 100 µl
SKU: 8472parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

HNF4α (phospho-Ser304)

available sizes

100 µL

Available product – rabbit anti-HNF4 α (phospho-Ser304) polyclonal antibody 8472

antibody
Tested applications
WB
Recommended dilutions
Immunoblotting: use at dilution of 1:1,000. A band of ~52kDa is detected.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications
Immunogen
Peptide sequence that includes phosphorylation site of Serine 304 (L-R-S(p)-Q-V) derived from human HNF4a and conjugated to KLH.
Size and concentration
100µL and 1 mg/mL
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C.
Storage buffer
PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl,
Purity
affinity purified
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Hepatocyte nuclear factor 4-alpha (HNF-4-alpha) (Nuclear receptor subfamily 2 group A member 1) (Transcription factor 14) (TCF-14) (Transcription factor HNF-4)
Gene names
HNF4A,HNF4A HNF4 NR2A1 TCF14
Protein family
Nuclear hormone receptor family, NR2 subfamily
Mass
52785Da
Function
FUNCTION: Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes (PubMed:30597922). Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells (PubMed:30530698). {ECO:0000250|UniProtKB:P49698, ECO:0000269|PubMed:30530698, ECO:0000269|PubMed:30597922}.
Subellular location
SUBCELLULAR LOCATION: Nucleus.
Structure
SUBUNIT: Homodimerization is required for HNF4-alpha to bind to its recognition site (PubMed:14982928). Interacts with CLOCK, BMAL1, CRY1, CRY2, PER1 and PER2 (PubMed:30530698). Interacts with NR0B2/SHP; the resulting heterodimer is transcriptionally inactive (PubMed:28128295). Interacts with DDX3X; this interaction disrupts the interaction between HNF4 and NR0B2 that forms inactive heterodimers and enhances the formation of active HNF4 homodimers (PubMed:28128295). {ECO:0000269|PubMed:14982928, ECO:0000269|PubMed:28128295, ECO:0000269|PubMed:30530698}.
Post-translational modification
PTM: Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA. {ECO:0000269|PubMed:12740371, ECO:0000269|PubMed:21708125, ECO:0000269|PubMed:7568236}.; PTM: Acetylation at Lys-458 lowers transcriptional activation by about two-fold. {ECO:0000269|PubMed:21708125}.
Target Relevance information above includes information from UniProt accession : P41235
The UniProt Consortium

Data

No results found

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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