rabbit anti-HNF4 α (phospho-Ser304) polyclonal antibody 8472

$366.00

Antibody summary

Rabbit polyclonal to HNF4 α (phospho-Ser304)
Suitable for: WB
Isotype: Whole IgG
100 µl

SKU: 8472parent Category: antibody Tags: HNF4 α (phospho-Ser304), polyclonal, rabbit

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	HNF4α (phospho-Ser304)
available sizes	100 µL

rabbit anti-HNF4 α (phospho-Ser304) polyclonal antibody 8472

antibody

Tested applications WB
Recommended dilutions Immunoblotting: use at dilution of 1:1,000. A band of ~52kDa is detected. These are recommended working dilutions. End user should determine optimal dilutions for their applications
Immunogen Peptide sequence that includes phosphorylation site of Serine 304 (L-R-S(p)-Q-V) derived from human HNF4a and conjugated to KLH.
Size and concentration 100µL and 1 mg/mL
Form liquid
Storage Instructions This antibody is stable for at least one (1) year at -20°C.
Storage buffer PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl,
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Hepatocyte nuclear factor 4-alpha (HNF-4-alpha) (Nuclear receptor subfamily 2 group A member 1) (Transcription factor 14) (TCF-14) (Transcription factor HNF-4)
Gene names HNF4A,HNF4A HNF4 NR2A1 TCF14
Protein family Nuclear hormone receptor family, NR2 subfamily
Mass 52785Da
Function FUNCTION: Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes (PubMed:30597922). Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells (PubMed:30530698). {ECO:0000250\|UniProtKB:P49698, ECO:0000269\|PubMed:30530698, ECO:0000269\|PubMed:30597922}.
Subellular location SUBCELLULAR LOCATION: Nucleus.
Structure SUBUNIT: Homodimerization is required for HNF4-alpha to bind to its recognition site (PubMed:14982928). Interacts with CLOCK, BMAL1, CRY1, CRY2, PER1 and PER2 (PubMed:30530698). Interacts with NR0B2/SHP; the resulting heterodimer is transcriptionally inactive (PubMed:28128295). Interacts with DDX3X; this interaction disrupts the interaction between HNF4 and NR0B2 that forms inactive heterodimers and enhances the formation of active HNF4 homodimers (PubMed:28128295). {ECO:0000269\|PubMed:14982928, ECO:0000269\|PubMed:28128295, ECO:0000269\|PubMed:30530698}.
Post-translational modification PTM: Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA. {ECO:0000269\|PubMed:12740371, ECO:0000269\|PubMed:21708125, ECO:0000269\|PubMed:7568236}.; PTM: Acetylation at Lys-458 lowers transcriptional activation by about two-fold. {ECO:0000269\|PubMed:21708125}.
Target Relevance information above includes information from UniProt accession : P41235
The UniProt Consortium

Data

No results found

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.