| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| available sizes | 1 mg, 100 µg |
rabbit anti-HA tag polyclonal antibody 6150
$426.00 – $2,415.00
Antibody summary
- Rabbit polyclonal to HA tag
- Suitable for: WB, ICC/IF, IP, ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG
- 100 µg, 1 mg
rabbit anti-HA tag polyclonal antibody 6150
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:20,000. Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500 These are |
| Immunogen HA (YPYDVPDYA) conjugated to KLH. |
| Size and concentration 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS, pH 7.2, 0.09% NaN3. |
| Purity peptide affinty purifcation |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Biotechnology The HA tag, derived from the human influenza hemagglutinin protein, is a widely employed tool in recombinant protein expression and has become an invaluable asset in molecular biology research. When incorporated at the N- or C-terminus of a target protein, the HA tag serves as a short, immunogenic peptide sequence, enabling efficient detection, purification, and characterization of the expressed protein. Its small size minimizes disruption to the target protein???s structure and function, making it suitable for a diverse range of applications. The HA tag is specifically recognized by commercially available high-affinity antibodies, facilitating the precise and sensitive detection of the tagged protein in techniques like Western blotting and immunofluorescence. Additionally, the HA tag is compatible with affinity chromatography, enabling straightforward and effective purification of the tagged protein from complex cellular extracts. This versatility has made the HA tag a valuable tool for studying protein interactions, localization, and post-translational modifications, providing researchers with valuable insights into cellular processes and molecular mechanisms across various fields of biology and biotechnology. |
Data
 |
| Detection of HA-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.Antibody: Affinity purified rabbit anti-HA antibody 6150 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
|
| Detection of HA-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.Antibody: Affinity purified rabbit anti-HA antibody 6150 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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