| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| reactivity | Glutathione-S-Transferase (GST) |
| available sizes | 100 µg |
rabbit anti-Glutathione-S-Transferase (GST) polyclonal antibody 9600
$271.00
Antibody summary
- Rabbit polyclonal to Glutathione-S-Transferase (GST)
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: IgG
- 100 µg
rabbit anti-Glutathione-S-Transferase (GST) polyclonal antibody 9600
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:20,000. Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500 These are |
| Immunogen Glutathione-S-Transferase (GST) from Schistosoma japonicum. |
| Size and concentration 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS, pH 7.2, 0.09% NaN3. |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Schistosoma japonicum GST28 Glutathione S-transferase class-mu 28 kDa isozyme |
| Protein names Glutathione S-transferase class-mu 28 kDa isozyme |
| Alternative names Sj28 antigen, Sj28GST |
| Protein family Belongs to the GST superfamily. Mu family |
| Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles |
| Catalytic activity RX + glutathione = an S-substituted glutathione + a halide anion + H(+) |
| Structure Homodimer |
| Keywords Transferase |
| Sequence VKLIYFNGRGRAEPIRMILVAAGVEFEDERIEFQDWPKIKPTIPGGRLPIVKITDKRGDV KTMSESLAIARFIARKHNMMGDTDDEYYIIEKMIGQVEDVESEYHKTLIKPPEEKEKISK EILNGKVPILLQAICETLKESTGNLTVGDKVTLADVVLIASIDHITDLDKEFLTGKYPEI HKHRKHLLATSPKLAKYLSERHATAF |
| UniProt accession: P26624 |
Data
 |
| Detection of GST-tagged Protein by western blot. Samples: 0.1, 0.3, or 1.0.µg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity purified, rabbit anti-GST antibody 9600 used for WB at 0.06 µg /ml (1:15,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
|
| Detection of GST-tagged Protein by western blot. Samples: 0.1, 0.3, or 1.0.µg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity purified, rabbit anti-GST antibody 9600 used for WB at 0.06 µg /ml (1:15,000). Detection: Chemiluminescence with an exposure time of 3 seconds. |
FAQ & Publications
Frequently Asked Questions
What applications has the rabbit anti-Glutathione-S-Transferase (GST) polyclonal antibody 9600 been validated for?
This antibody is suitable and has been tested for use in Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), enzyme-linked immunosorbent assay (ELISA), and immunoprecipitation (IP).
How should the rabbit anti-GST antibody 9600 be stored to maintain stability?
The antibody should be stored at 2-8°C, where it remains stable for at least one year.
What is the recommended dilution range for using this antibody in immunoblotting?
For immunoblotting (Western blot), the recommended dilution range is 1:1,000 to 1:20,000.
What is the host species and clonality of the anti-Glutathione-S-Transferase antibody 9600?
The antibody is a rabbit polyclonal antibody with an IgG isotype.
What is the immunogen used to generate the rabbit anti-GST polyclonal antibody 9600?
The immunogen is Glutathione-S-Transferase (GST) derived from Schistosoma japonicum.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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