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rabbit anti-Glutathione-S-Transferase (GST) polyclonal antibody 9600

$271.00

Antibody summary

  • Rabbit polyclonal to Glutathione-S-Transferase (GST)
  • Suitable for: WB,IHC,ICC,ELISA
  • Isotype: IgG
  • 100 µg
SKU: 9600parent Category: Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ELISA, ICC/IF, WB

reactivity

Glutathione-S-Transferase (GST)

available sizes

100 µg

rabbit anti-Glutathione-S-Transferase (GST) polyclonal antibody 9600

antibody
Tested applications
WB,ICC/IF,ELISA,IP
Recommended dilutions
Immunoblotting: use at a dilution of 1:1,000- 1:20,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

Immunoprecipitation: use 1-4ug/mg lysate.

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500

These are
Immunogen
Glutathione-S-Transferase (GST) from Schistosoma japonicum.
Size and concentration
100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at 2-8°C.
Storage buffer
PBS, pH 7.2, 0.09% NaN3.
Purity
affinity purified
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Glutathione S-transferase class-mu 28 kDa isozyme (GST 28) (EC 2.5.1.18) (Sj28 antigen) (Sj28GST)
Protein family
GST superfamily, Mu family
Mass
23393Da
Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.; GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Catalytic activity
CATALYTIC ACTIVITY: Reaction=glutathione + RX = a halide anion + an S-substituted glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925, ChEBI:CHEBI:90779; EC=2.5.1.18;
Structure
Homodimer.
Target Relevance information above includes information from UniProt accession: P26624
The UniProt Consortium

Data

benchmark-antibodies_anti-glutathione-s-transferase_gst_antibody_9600_1
Detection of GST-tagged Protein by western blot. Samples: 0.1, 0.3, or 1.0.µg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity purified, rabbit anti-GST antibody 9600 used for WB at 0.06 µg /ml (1:15,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
benchmark-antibodies_anti-glutathione-s-transferase_gst_antibody_9600_1
Detection of GST-tagged Protein by western blot. Samples: 0.1, 0.3, or 1.0.µg of 293 cell lysate expressing a GST-tag fusion protein. Antibodies: Affinity purified, rabbit anti-GST antibody 9600 used for WB at 0.06 µg /ml (1:15,000). Detection: Chemiluminescence with an exposure time of 3 seconds.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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