Skip to content

rabbit anti-GATA1 (phospho-Ser310) polyclonal antibody 8063

$366.00

Antibody summary

  • Rabbit polyclonal to GATA1 (phospho-Ser310)
  • Suitable for: WB,IF
  • Isotype: Whole IgG
  • 100 µl
SKU: 8063parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

GATA1 (phospho-Ser310)

available sizes

100 µL

Available product – rabbit anti-GATA1 (phospho-Ser310) polyclonal antibody 8063

antibody
Tested applications
WB,ICC/IF
Recommended dilutions
Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~43kDa is detected.

Immunofluorescence: use at dilution of 1:100-1:200.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications
Immunogen
Peptide sequence that includes phosphorylation site of Serine 310 (K-A-S(p)-G-K) derived from human GATA1 and conjugated to KLH.
Size and concentration
100µL and 1 mg/mL
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C.
Storage buffer
PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl,
Purity
affinity purified
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Erythroid transcription factor (Eryf1) (GATA-binding factor 1) (GATA-1) (GF-1) (NF-E1 DNA-binding protein)
Gene names
GATA1,GATA1 ERYF1 GF1
Mass
42751Da
Function
FUNCTION: Transcriptional activator or repressor which serves as a general switch factor for erythroid development (PubMed:35030251). It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781). {ECO:0000269|PubMed:22235304, ECO:0000269|PubMed:24245781, ECO:0000269|PubMed:35030251}.
Subellular location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:35030251}.
Tissues
TISSUE SPECIFICITY: Erythrocytes. {ECO:0000269|PubMed:8524811}.
Structure
SUBUNIT: May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with BRD3 (By similarity). Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo (By similarity). Interacts with EP300. Interacts with MED1, CCAR1 and CALCOCO1. Interacts with CEBPE (PubMed:26019275). {ECO:0000250|UniProtKB:P17679, ECO:0000269|PubMed:10700180, ECO:0000269|PubMed:11418466, ECO:0000269|PubMed:11675338, ECO:0000269|PubMed:11809723, ECO:0000269|PubMed:12200364, ECO:0000269|PubMed:17420275, ECO:0000269|PubMed:24245781, ECO:0000269|PubMed:26019275, ECO:0000269|PubMed:8524811, ECO:0000269|PubMed:9859997}.
Post-translational modification
PTM: Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137 (By similarity). {ECO:0000250}.; PTM: Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity (By similarity). {ECO:0000250}.; PTM: Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo (By similarity). Acetylated on Lys-233, Lys-245 Lys-246 by EP300. {ECO:0000250, ECO:0000269|PubMed:9859997}.
Target Relevance information above includes information from UniProt accession : P15976
The UniProt Consortium

Data

No results found

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

#
No results found

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.