rabbit anti-GATA1 (phospho-Ser310) polyclonal antibody 8063

$366.00

Antibody summary

Rabbit polyclonal to GATA1 (phospho-Ser310)
Suitable for: WB,IF
Isotype: Whole IgG
100 µl

SKU: 8063parent Category: antibody Tags: GATA1 (phospho-Ser310), polyclonal, rabbit

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	GATA1 (phospho-Ser310)
available sizes	100 µL

rabbit anti-GATA1 (phospho-Ser310) polyclonal antibody 8063

antibody

Tested applications WB,ICC/IF
Recommended dilutions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~43kDa is detected. Immunofluorescence: use at dilution of 1:100-1:200. These are recommended working dilutions. End user should determine optimal dilutions for their applications
Immunogen Peptide sequence that includes phosphorylation site of Serine 310 (K-A-S(p)-G-K) derived from human GATA1 and conjugated to KLH.
Size and concentration 100µL and 1 mg/mL
Form liquid
Storage Instructions This antibody is stable for at least one (1) year at -20°C.
Storage buffer PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl,
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Erythroid transcription factor (Eryf1) (GATA-binding factor 1) (GATA-1) (GF-1) (NF-E1 DNA-binding protein)
Gene names GATA1,GATA1 ERYF1 GF1
Mass 42751Da
Function FUNCTION: Transcriptional activator or repressor which serves as a general switch factor for erythroid development (PubMed:35030251). It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781). {ECO:0000269\|PubMed:22235304, ECO:0000269\|PubMed:24245781, ECO:0000269\|PubMed:35030251}.
Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000269\|PubMed:35030251}.
Tissues TISSUE SPECIFICITY: Erythrocytes. {ECO:0000269\|PubMed:8524811}.
Structure SUBUNIT: May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with BRD3 (By similarity). Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo (By similarity). Interacts with EP300. Interacts with MED1, CCAR1 and CALCOCO1. Interacts with CEBPE (PubMed:26019275). {ECO:0000250\|UniProtKB:P17679, ECO:0000269\|PubMed:10700180, ECO:0000269\|PubMed:11418466, ECO:0000269\|PubMed:11675338, ECO:0000269\|PubMed:11809723, ECO:0000269\|PubMed:12200364, ECO:0000269\|PubMed:17420275, ECO:0000269\|PubMed:24245781, ECO:0000269\|PubMed:26019275, ECO:0000269\|PubMed:8524811, ECO:0000269\|PubMed:9859997}.
Post-translational modification PTM: Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137 (By similarity). {ECO:0000250}.; PTM: Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity (By similarity). {ECO:0000250}.; PTM: Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo (By similarity). Acetylated on Lys-233, Lys-245 Lys-246 by EP300. {ECO:0000250, ECO:0000269\|PubMed:9859997}.
Target Relevance information above includes information from UniProt accession : P15976
The UniProt Consortium

Data

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Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.