Weight | 1 lbs |
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Dimensions | 9 × 5 × 2 in |
host | rabbit |
isotype | IgG |
clonality | polyclonal |
concentration | 1 mg/mL |
applications | ICC/IF, WB |
reactivity | FLIP (NT) |
available sizes | 100 µg |
rabbit anti-FLIP (NT) polyclonal antibody 3183
$445.00
Antibody summary
- Rabbit polyclonal to FLIP (NT)
- Suitable for: ELISA,WB,IHC-P,ICC,IF,FC
- Isotype: IgG
- 100 µg
rabbit anti-FLIP (NT) polyclonal antibody 3183
antibody |
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Tested applications WB,IHC,IHC,ICC/IF,ELISA |
Recommended dilutions Immunoblotting : use at 1-2ug/mL Positive control: K562 cell lysate. Immunocytochemistry: use at 5ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. |
Immunogen Peptide corresponding to aa 2-18 of human FLIP (accession no. AAC51622). This sequence is identical in all FLIP splice variants. |
Size and concentration 100µg and lot specific |
Form liquid |
Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
Storage buffer PBS, pH 7.4. |
Purity peptide affinty purifcation |
Clonality polyclonal |
Isotype IgG |
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
Isotype control Rabbit polyclonal - Isotype Control |
target relevance |
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Protein names CASP8 and FADD-like apoptosis regulator (Caspase homolog) (CASH) (Caspase-eight-related protein) (Casper) (Caspase-like apoptosis regulatory protein) (CLARP) (Cellular FLICE-like inhibitory protein) (c-FLIP) (FADD-like antiapoptotic molecule 1) (FLAME-1) (Inhibitor of FLICE) (I-FLICE) (MACH-related inducer of toxicity) (MRIT) (Usurpin) [C |
Gene names CFLAR,CFLAR CASH CASP8AP1 CLARP MRIT |
Protein family Peptidase C14A family |
Mass 55344Da |
Function Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity. |
Tissues Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. |
Structure TNFRSF6 stimulation triggers recruitment to the death-inducing signaling complex (DISC) formed by TNFRSF6, FADD and CASP8 (PubMed:9880531). A proteolytic fragment (p43) stays associated with the DISC (PubMed:9880531). Also interacts with FADD, CASP8, CASP3, TRAF1, TRAF2 and Bcl-X(L) (in vitro) (PubMed:9326610, PubMed:9208847). Interacts with RIPK1 (By similarity) (PubMed:9326610, PubMed:9208847, PubMed:9880531).; (Microbial infection) Interacts with HBV protein X. |
Post-translational modification Proteolytically processed by CASP8 generating subunit p43 and p12. |
Target Relevance information above includes information from UniProt accession: O15519 |
The UniProt Consortium |
Publications
pmid | title | authors | citation |
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We haven't added any publications to our database yet. |
relevant to this product |
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Western blot IHC ICC |
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