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rabbit anti-F1A α (CT) polyclonal antibody 8308

$445.00

Antibody summary

  • Rabbit polyclonal to F1A α (CT)
  • Suitable for: ELISA,WB,IHC-P,IF
  • Isotype: IgG
  • 100 µg
SKU: 8308parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

F1Aa

available sizes

100 µg

Available product – rabbit anti-F1A α (CT) polyclonal antibody 8308

antibody
Tested applications
WB,IHC,IHC,ICC/IF,ELISA
Recommended dilutions
Immunoblotting: use at 1ug/mL.

Immunohistochemistry: use at 5ug/mL.

Positive control: Mouse liver tissue lysate.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
Immunogen
Peptide corresponding to aa 609- 622 of human F1A (accession no. AAF05314). This sequence is identical to that of mouse.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Protein fem-1 homolog B (FEM1b) (FEM1-beta) (Fem-1-like death receptor-binding protein alpha) (Fem-1-like in apoptotic pathway protein alpha) (F1A-alpha)
Gene names
FEM1B,FEM1B F1AA KIAA0396
Protein family
Fem-1 family
Mass
70264Da
Function
FUNCTION: Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:29779948, PubMed:33398170, PubMed:33398168). The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms (PubMed:29779948, PubMed:33398170, PubMed:33398168). The CRL2(FEM1B) complex specifically recognizes proteins ending with -Gly-Leu-Asp-Arg, such as CDK5R1, leading to their ubiquitination and degradation (PubMed:33398170, PubMed:33398168). Also acts as a regulator of the reductive stress response by mediating ubiquitination of reduced FNIP1: in response to reductive stress, the CRL2(FEM1B) complex specifically recognizes a conserved Cys degron in FNIP1 when this degron is reduced, leading to FNIP1 degradation and subsequent activation of mitochondria to recalibrate reactive oxygen species (ROS) (By similarity). Mechanistically, recognizes and binds reduced FNIP1 through two interface zinc ions, which act as a molecular glue that recruit reduced FNIP1 to FEM1B (By similarity). Promotes ubiquitination of GLI1, suppressing GLI1 transcriptional activator activity (PubMed:24076122). Promotes ubiquitination and degradation of ANKRD37 (By similarity). Promotes ubiquitination and degradation of SLBP (PubMed:28118078). Involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis (PubMed:10542291). Also involved in glucose homeostasis in pancreatic islet (By similarity). May also act as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1 (PubMed:19330022). {ECO:0000250|UniProtKB:Q9Z2G0, ECO:0000269|PubMed:10542291, ECO:0000269|PubMed:19330022, ECO:0000269|PubMed:24076122, ECO:0000269|PubMed:28118078, ECO:0000269|PubMed:29779948, ECO:0000269|PubMed:33398168, ECO:0000269|PubMed:33398170}.
Pathway
PATHWAY: Protein modification; protein ubiquitination. {ECO:0000269|PubMed:24076122, ECO:0000269|PubMed:28118078, ECO:0000269|PubMed:29779948, ECO:0000269|PubMed:33398168, ECO:0000269|PubMed:333981
Subellular location
SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19330022, ECO:0000269|PubMed:24076122}. Nucleus {ECO:0000269|PubMed:19330022}. Note=In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment (PubMed:19330022). Associated with chromatin (PubMed:19330022). {ECO:0000269|PubMed:19330022}.
Tissues
TISSUE SPECIFICITY: Widely expressed (PubMed:10542291). Highly expressed in testis (PubMed:10542291). Weakly expressed in other tissues (PubMed:10542291). {ECO:0000269|PubMed:10542291}.
Structure
SUBUNIT: Component of a CRL2 E3 ubiquitin-protein ligase complex, also named ECS (Elongin BC-CUL2/5-SOCS-box protein) complex, composed of CUL2, Elongin BC (ELOB and ELOC), RBX1 and substrate-specific adapter FEM1B (PubMed:15601820, PubMed:29779948). Homooligomer (PubMed:10542291). Interacts with PPM1F and PHTF1 (PubMed:11559703). Interacts with the death domain of FAS/TNFRSF6 and TNFRSF1A (PubMed:10542291). Interacts with CHEK1 (PubMed:19330022). Interacts with NKX3-1 (By similarity). {ECO:0000250|UniProtKB:Q9Z2G0, ECO:0000269|PubMed:10542291, ECO:0000269|PubMed:11559703, ECO:0000269|PubMed:15601820, ECO:0000269|PubMed:19330022, ECO:0000269|PubMed:29779948}.
Target Relevance information above includes information from UniProt accession : Q9UK73
The UniProt Consortium

Data

benchmark-antibodies_anti-f1aa_antibody_8308_1.jpg
Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 µg/mL.
benchmark-antibodies_anti-f1aa_antibody_8308_2.jpg
Immunohistochemistry of F1Aα in mouse liver tissue with F1Aα antibody at 5 µg/mL.
benchmark-antibodies_anti-f1aa_antibody_8308_3.jpg
Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha antibody at 20 µg/mL.
benchmark-antibodies_anti-f1aa_antibody_8308_4.gif
Immunofluorescence of F1A alpha in mouse liver tissue with F1A alpha antibody at 20 µg/mL. Green: F1A alpha Antibody (8308)
Blue: DAPI staining

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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