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rabbit anti-DR5 polyclonal antibody 9289

$445.00

Antibody summary

  • Rabbit polyclonal to DR5
  • Suitable for: ELISA,WB,IHC-P,IF
  • Isotype: IgG
  • 100 µg
SKU: 9289parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

DR5

available sizes

100 µg

Available product – rabbit anti-DR5 polyclonal antibody 9289

antibody
Tested applications
WB,IHC,IHC,ICC/IF,ELISA
Recommended dilutions
Immunoblotting : use at 2ug/mL.

Immunocytochemistry: use at 5ug/mL.

Positive control: Whole cell lysate from HeLa cells or K562 cells.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
Immunogen
Peptide corresponding to aa 388- 407 of human DR5 precursor (accession no. AF012535).
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Tumor necrosis factor receptor superfamily member 10B (Death receptor 5) (TNF-related apoptosis-inducing ligand receptor 2) (TRAIL receptor 2) (TRAIL-R2) (CD antigen CD262)
Gene names
TNFRSF10B,TNFRSF10B DR5 KILLER TRAILR2 TRICK2 ZTNFR9 UNQ160/PRO186
Mass
47878Da
Function
FUNCTION: Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed:10549288). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. {ECO:0000269|PubMed:10542098, ECO:0000269|PubMed:10549288, ECO:0000269|PubMed:15322075}.
Subellular location
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
Tissues
TISSUE SPECIFICITY: Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.
Structure
SUBUNIT: Monomer (PubMed:10549288). Can interact with TRADD and RIPK1. Interacts with HCMV protein UL141; this interaction prevents TNFRSF10B cell surface expression. Two TNFRSF10B monomers interact with a UL141 homodimer. Three TNFRSF10B molecules interact with TNFSF10 homotrimer (PubMed:10549288). In the absence of stimulation, interacts with BIRC2, DDX3X and GSK3B. The interaction with BIRC2 and DDX3X is further enhanced upon receptor stimulation and accompanied by DDX3X and BIRC2 cleavage (PubMed:18846110). {ECO:0000269|PubMed:10549288, ECO:0000269|PubMed:18846110, ECO:0000269|PubMed:23498957, ECO:0000269|PubMed:23555243}.
Post-translational modification
PTM: (Microbial infection) Glycosylated on Arg residue by S.typhimurium protein Ssek3. {ECO:0000269|PubMed:30902834}.
Domain
TOPO_DOM 5
Target Relevance information above includes information from UniProt accession : O14763
The UniProt Consortium

Data

benchmark-antibodies_anti-dr5_antibody_9289_1.gif
Western Blot Validation in Human Cell Lines
Loading: 15 µg of lysates per lane. Antibodies: DR5 9289, (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
benchmark-antibodies_anti-dr5_antibody_9289_2.gif
Western Blot Validation in Human HepG2 Cells
Loading: 15 µg of lysates per lane. Antibodies: DR5 9289, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 µg/mLLane 2: 2 µg/mLLane 3: 4 µg/mL
benchmark-antibodies_anti-dr5_antibody_9289_3.gif
Western Blot Validation in Mouse and Rat Cell Lines
Loading: 15 µg of lysates per lane. Antibodies: DR5 9289, (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP
benchmark-antibodies_anti-dr5_antibody_9289_4.gif
Western Blot Validation in Mouse Cell Lines
Loading: 15 µg of lysates per lane. Antibodies: DR5 9289, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
benchmark-antibodies_anti-dr5_antibody_9289_5.gif
Western Blot Validation in Mouse Heart
Loading: 15 µg of lysatesper lane. Antibodies: DR5 9289, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
benchmark-antibodies_anti-dr5_antibody_9289_6.gif
Western Blot Validation in Rat Skeletal Muscle
Loading: 15 µg of lysate per lane. Antibodies: DR5 9289, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
benchmark-antibodies_anti-dr5_antibody_9289_7.jpg
Immunofluorescence Validation of DR5 in Human HepG2 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed human HepG2 cells labeling DR5 with 9289 at 5 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI (blue).
benchmark-antibodies_anti-dr5_antibody_9289_8.jpg
Immunofluorescence Validation of DR5 in Human Testis
Immunofluorescent analysis of 4% paraformaldehyde-fixed human testis tissue labeling DR5 with 9289 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI (blue).
benchmark-antibodies_anti-dr5_antibody_9289_9.jpg
Immunofluorescence Validation of DR5 in Mouse Pancreas
Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling DR5 with 9289 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI (blue).

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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