Weight | 1 lbs |
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Dimensions | 9 × 5 × 2 in |
host | rabbit |
isotype | IgG |
clonality | polyclonal |
concentration | 1 mg/mL |
applications | ICC/IF, WB |
reactivity | DR4 (NT) |
available sizes | 100 µg |
rabbit anti-DR4 (NT) polyclonal antibody 6001
$445.00
Antibody summary
- Rabbit polyclonal to DR4 (NT)
- Suitable for: ELISA,WB,IF,ICC
- Isotype: IgG
- 100 µg
rabbit anti-DR4 (NT) polyclonal antibody 6001
antibody |
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Tested applications WB,IHC,IHC,ICC/IF,ELISA |
Recommended dilutions Immunoblotting : use at 2ug/mL. Positive control: Whole cell lysate from HeLa, K562, or Jurkat cells. These are recommended concentrations. Enduser should determine optimal concentration for their applications. |
Immunogen Peptide corresponding to aa 1-20 of human DR4 mature protein (accession no. ACC51226). |
Size and concentration 100µg and lot specific |
Form liquid |
Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
Storage buffer PBS, pH 7.4. |
Purity peptide affinty purifcation |
Clonality polyclonal |
Isotype IgG |
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
Isotype control Rabbit polyclonal - Isotype Control |
target relevance |
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Protein names Tumor necrosis factor receptor superfamily member 10A (Death receptor 4) (TNF-related apoptosis-inducing ligand receptor 1) (TRAIL receptor 1) (TRAIL-R1) (CD antigen CD261) |
Gene names TNFRSF10A,TNFRSF10A APO2 DR4 TRAILR1 |
Mass 50089Da |
Function Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed:26457518). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis (PubMed:19090789). Promotes the activation of NF-kappa-B (PubMed:9430227). |
Subellular location Cell membrane ; Single-pass type I membrane protein. Membrane raft. Cytoplasm, cytosol. Note=Palmitoylation is required for association with membranes. |
Tissues Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells. |
Structure Monomer (PubMed:26457518). Homooligomers and heterooligomers with TNFRSF10B (PubMed:19090789). Three TNFRSF10A molecules interact with the TNFSF10 homotrimer (PubMed:26457518). Can interact with TRADD and RIPK1. Interacts with ARAP1. In the absence of stimulation, interacts with BIRC2, DDX3X and GSK3B. The interaction with BIRC2 and DDX3X is further enhanced upon receptor stimulation and accompanied by DDX3X and BIRC2 cleavage (PubMed:18846110). Interacts with ZDHHC3 (PubMed:22240897).; (Microbial infection) Interacts with HCMV protein UL141; this interaction prevents TNFRSF10A cell surface expression. |
Post-translational modification Palmitoylated (PubMed:19090789). Palmitoylation of TNFRSF10A is required for its association with lipid rafts, oligomerization and function in TRAIL-induced cell death (PubMed:19090789). Palmitoylated by ZDHHC3 (Probable). |
Domain TOPO_DOM 2 |
Target Relevance information above includes information from UniProt accession: O00220 |
The UniProt Consortium |
Publications
pmid | title | authors | citation |
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We haven't added any publications to our database yet. |
relevant to this product |
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Western blot IHC ICC |
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