| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | DR3 (ED) |
| available sizes | 100 µg |
rabbit anti-DR3 (ED) polyclonal antibody 1563
$445.00
Antibody summary
- Rabbit polyclonal to DR3 (ED)
- Suitable for: ELISA,WB,ICC,IF
- Isotype: IgG
- 100 µg
rabbit anti-DR3 (ED) polyclonal antibody 1563
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at 1ug/mL. Immunocytochemistry: use at 10ug/mL. Positive control: Whole cell lysate from Jurkat cells. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. |
| Immunogen Peptide corresponding to the extracellular domain of human DR3 precursor (accession no. AAQ88676). |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Tumor necrosis factor receptor superfamily member 25 (Apo-3) (Apoptosis-inducing receptor AIR) (Apoptosis-mediating receptor DR3) (Apoptosis-mediating receptor TRAMP) (Death receptor 3) (Lymphocyte-associated receptor of death) (LARD) (Protein WSL) (Protein WSL-1) |
| Gene names TNFRSF25,TNFRSF25 APO3 DDR3 DR3 TNFRSF12 WSL WSL1 UNQ455/PRO779 |
| Mass 45385Da |
| Function FUNCTION: Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. {ECO:0000269|PubMed:8875942, ECO:0000269|PubMed:8994832, ECO:0000269|PubMed:9052839}. |
| Subellular location SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 9]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 11]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 3]: Secreted.; SUBCELLULAR LOCATION: [Isoform 4]: Secreted.; SUBCELLULAR LOCATION: [Isoform 5]: Secreted.; SUBCELLULAR LOCATION: [Isoform 6]: Secreted.; SUBCELLULAR LOCATION: [Isoform 7]: Secreted.; SUBCELLULAR LOCATION: [Isoform 8]: Secreted.; SUBCELLULAR LOCATION: [Isoform 10]: Secreted.; SUBCELLULAR LOCATION: [Isoform 12]: Secreted. |
| Tissues TISSUE SPECIFICITY: Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate. |
| Structure SUBUNIT: Homodimer. Interacts strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4. {ECO:0000269|PubMed:9915703}. |
| Post-translational modification PTM: (Microbial infection) Glycosylated at Arg-352 by enteropathogenic E.coli protein NleB1. {ECO:0000305|PubMed:30979585}.; PTM: Glycosylated. {ECO:0000305}. |
| Target Relevance information above includes information from UniProt accession: Q93038 |
| The UniProt Consortium |
Data
 |
| Western blot analysis of DR3 in Jurkat total cell lysate with DR3 antibody at 1:1000 dilution. |
 |
| Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 µg/mL. |
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| Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 µg/mL. |
 |
| Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 µg/mL.
Green: DR3 Antibody (1563) Blue: DAPI staining |
 |
| Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 µg/mL. |
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| # | SDS | Certificate | |
|---|---|---|---|
| Please enter your product and batch number here to retrieve product datasheet, SDS, and QC information. | |||
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