| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | DR3 (ED) |
| available sizes | 100 µg |
rabbit anti-DR3 (ED) polyclonal antibody 1563
$445.00
Antibody summary
- Rabbit polyclonal to DR3 (ED)
- Suitable for: ELISA,WB,ICC,IF
- Isotype: IgG
- 100 µg
rabbit anti-DR3 (ED) polyclonal antibody 1563
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at 1ug/mL. Immunocytochemistry: use at 10ug/mL. Positive control: Whole cell lysate from Jurkat cells. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. |
| Immunogen Peptide corresponding to the extracellular domain of human DR3 precursor (accession no. AAQ88676). |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Tumor necrosis factor receptor superfamily member 25 (Apo-3) (Apoptosis-inducing receptor AIR) (Apoptosis-mediating receptor DR3) (Apoptosis-mediating receptor TRAMP) (Death receptor 3) (Lymphocyte-associated receptor of death) (LARD) (Protein WSL) (Protein WSL-1) |
| Gene names TNFRSF25,TNFRSF25 APO3 DDR3 DR3 TNFRSF12 WSL WSL1 UNQ455/PRO779 |
| Mass 45385Da |
| Function FUNCTION: Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. {ECO:0000269|PubMed:8875942, ECO:0000269|PubMed:8994832, ECO:0000269|PubMed:9052839}. |
| Subellular location SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 9]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 11]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 3]: Secreted.; SUBCELLULAR LOCATION: [Isoform 4]: Secreted.; SUBCELLULAR LOCATION: [Isoform 5]: Secreted.; SUBCELLULAR LOCATION: [Isoform 6]: Secreted.; SUBCELLULAR LOCATION: [Isoform 7]: Secreted.; SUBCELLULAR LOCATION: [Isoform 8]: Secreted.; SUBCELLULAR LOCATION: [Isoform 10]: Secreted.; SUBCELLULAR LOCATION: [Isoform 12]: Secreted. |
| Tissues TISSUE SPECIFICITY: Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate. |
| Structure SUBUNIT: Homodimer. Interacts strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4. {ECO:0000269|PubMed:9915703}. |
| Post-translational modification PTM: (Microbial infection) Glycosylated at Arg-352 by enteropathogenic E.coli protein NleB1. {ECO:0000305|PubMed:30979585}.; PTM: Glycosylated. {ECO:0000305}. |
| Target Relevance information above includes information from UniProt accession: Q93038 |
| The UniProt Consortium |
Data
 |
| Western blot analysis of DR3 in Jurkat total cell lysate with DR3 antibody at 1:1000 dilution. |
 |
| Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 µg/mL. |
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| Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 µg/mL. |
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| Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 µg/mL.
Green: DR3 Antibody (1563) Blue: DAPI staining |
 |
| Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 µg/mL. |
FAQ & Publications
Frequently Asked Questions
What applications has the rabbit anti-DR3 (ED) polyclonal antibody 1563 been validated for?
This antibody has been tested and is suitable for ELISA, Western blot (WB), immunocytochemistry (ICC), and immunofluorescence (IF) applications.
How should the rabbit anti-DR3 (ED) polyclonal antibody 1563 be stored to maintain stability?
The antibody is stable for at least one year when stored at -20°C. It is recommended to avoid multiple freeze-thaw cycles to preserve antibody integrity.
What is the immunogen used to generate the rabbit anti-DR3 (ED) polyclonal antibody 1563?
The immunogen is a peptide corresponding to the extracellular domain of the human DR3 precursor, based on the sequence with accession number AAQ88676.
Which secondary antibodies are compatible with the rabbit anti-DR3 (ED) polyclonal antibody 1563 for detection?
Compatible secondary antibodies include goat anti-rabbit IgG (H&L chain specific) conjugated to peroxidase, biotin, or FITC, including cross-absorbed variants for reduced cross-reactivity.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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