ICC/IF rabbit anti dff40 cad i18 polyclonal antibody 2093

rabbit anti-DFF40/CAD (I18) polyclonal antibody 2093

$445.00

Antibody summary

Rabbit polyclonal to DFF40/CAD (I18)
Suitable for: ELISA,WB,ICC,IF
Isotype: IgG
100 µg

SKU: 2093parent Category: antibody Tags: DFF40/CAD (I18), polyclonal, rabbit

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	DFF40/CAD (I18)
available sizes	100 µg

rabbit anti-DFF40/CAD (I18) polyclonal antibody 2093

antibody

Tested applications WB,ICC/IF,ELISA
Recommended dilutions Immunoblotting: use at 1ug/mL Immunocytochemistry: use at 5ug/mL. Positive control: Whole cell lysate of K562 or Jurkat cells. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Immunogen Peptide corresponding to aa 147-164 near the center of murine CAD (accession no. NP_031885). The sequence differs from human DFF40 by two amino acids.
Size and concentration 100µg and lot specific
Form liquid
Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer PBS, pH 7.4.
Purity peptide affinty purifcation
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names DNA fragmentation factor subunit beta (EC 3.-.-.-) (Caspase-activated deoxyribonuclease) (CAD) (Caspase-activated DNase) (DNA fragmentation factor 40 kDa subunit) (DFF-40)
Gene names Dffb,Dffb Cad
Mass 39449Da
Function FUNCTION: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Subellular location SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
Structure SUBUNIT: Heterodimer of DFFA and DFFB (By similarity). Interacts with H1-1 (By similarity). {ECO:0000250\|UniProtKB:O76075}.
Target Relevance information above includes information from UniProt accession : O54788
The UniProt Consortium

Data

benchmark-antibodies_anti-dff40-cad_i18_antibody_2093_1.jpg

Western blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40 antibody (I18) at 1:500 dilution.

benchmark-antibodies_anti-dff40-cad_i18_antibody_2093_2.jpg

Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 µg/mL.

benchmark-antibodies_anti-dff40-cad_i18_antibody_2093_3.gif

Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 µg/mL.

benchmark-antibodies_anti-dff40-cad_i18_antibody_2093_4.gif

Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 µg/mL. Green: DFF40 Antibody (2093)
Blue: DAPI staining

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.