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rabbit anti-DFF40/CAD (I18) polyclonal antibody 2093

$445.00

Antibody summary

  • Rabbit polyclonal to DFF40/CAD (I18)
  • Suitable for: ELISA,WB,ICC,IF
  • Isotype: IgG
  • 100 µg
SKU: 2093parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

DFF40/CAD (I18)

available sizes

100 µg

Available product – rabbit anti-DFF40/CAD (I18) polyclonal antibody 2093

antibody
Tested applications
WB,ICC/IF,ELISA
Recommended dilutions
Immunoblotting: use at 1ug/mL

Immunocytochemistry: use at 5ug/mL.

Positive control: Whole cell lysate of K562 or Jurkat cells.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
Immunogen
Peptide corresponding to aa 147-164 near the center of murine CAD (accession no. NP_031885). The sequence differs from human DFF40 by two amino acids.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
DNA fragmentation factor subunit beta (EC 3.-.-.-) (Caspase-activated deoxyribonuclease) (CAD) (Caspase-activated DNase) (DNA fragmentation factor 40 kDa subunit) (DFF-40)
Gene names
Dffb,Dffb Cad
Mass
39449Da
Function
FUNCTION: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Subellular location
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
Structure
SUBUNIT: Heterodimer of DFFA and DFFB (By similarity). Interacts with H1-1 (By similarity). {ECO:0000250|UniProtKB:O76075}.
Target Relevance information above includes information from UniProt accession : O54788
The UniProt Consortium

Data

benchmark-antibodies_anti-dff40-cad_i18_antibody_2093_1.jpg
Western blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40 antibody (I18) at 1:500 dilution.
benchmark-antibodies_anti-dff40-cad_i18_antibody_2093_2.jpg
Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 µg/mL.
benchmark-antibodies_anti-dff40-cad_i18_antibody_2093_3.gif
Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 µg/mL.
benchmark-antibodies_anti-dff40-cad_i18_antibody_2093_4.gif
Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 µg/mL. Green: DFF40 Antibody (2093)
Blue: DAPI staining

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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