| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | DFF40/CAD (NT) |
| available sizes | 100 µg |
rabbit anti-DFF40 (NT) polyclonal antibody 6548
$445.00
Antibody summary
- Rabbit polyclonal to DFF40 (NT)
- Suitable for: ELISA,WB,ICC,IF
- Isotype: IgG
- 100 µg
rabbit anti-DFF40 (NT) polyclonal antibody 6548
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at 2ug/mL. Immunocytochemistry: use at 5ug/mL. Positive control: Whole cell lysate of K562 or Jurkat cells. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. |
| Immunogen Peptide corresponding to aa 3-18 of human DFF40 (accession no. NP_004393). |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinty purifcation |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names DNA fragmentation factor subunit beta (EC 3.-.-.-) (Caspase-activated deoxyribonuclease) (CAD) (Caspase-activated DNase) (DNA fragmentation factor 40 kDa subunit) (DFF-40) |
| Gene names Dffb,Dffb Cad |
| Mass 39449Da |
| Function Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. |
| Subellular location Cytoplasm. Nucleus. |
| Structure Heterodimer of DFFA and DFFB (By similarity). Interacts with H1-1 (By similarity). |
| Target Relevance information above includes information from UniProt accession: O54788 |
| The UniProt Consortium |
 |
| Western blot analysis of DFF40 in Jurkat (J) and K562 (K) whole cell lysate with DFF40 antibody at 1:500 dilution. |
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| Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 µg/mL. |
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| Immunofluorescence of DFF40 in Jurkat cells with DFF40 antibody at 20 µg/mL. |
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
| relevant to this product |
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| Western blot IHC ICC |
| # | |||
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| Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information. | |||
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