| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | DFF (CT) |
| available sizes | 100 µg |
rabbit anti-DFF (CT) BP polyclonal antibody 6651
$445.00
Antibody summary
- Rabbit polyclonal to DFF (CT) BP
- Suitable for: ELISA,WB,IHC-P,IP,IF
- Isotype: IgG
- 100 µg
rabbit anti-DFF (CT) BP polyclonal antibody 6651
| antibody |
|---|
| Tested applications WB,IHC,IHC,ELISA,IP |
| Recommended dilutions Immunoblotting: use at 1ug/mL. Immunocytochemistry: use at 5ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Whole cell lysate from HeLa cells. |
| Immunogen Peptide corresponding to aa 313-331 at the C-terminus of human DFF45 (accession no. NP_004392). |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinty purifcation |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names DNA fragmentation factor subunit alpha (DNA fragmentation factor 45 kDa subunit) (DFF-45) (Inhibitor of CAD) (ICAD) |
| Gene names DFFA,DFFA DFF1 DFF45 H13 |
| Mass 36522Da |
| Function Inhibitor of the caspase-activated DNase (DFF40). |
| Subellular location Cytoplasm. |
| Structure Heterodimer of DFFA and DFFB. |
| Post-translational modification Caspase-3 cleaves DFF45 at 2 sites to generate an active factor. |
| Target Relevance information above includes information from UniProt accession: O00273 |
| The UniProt Consortium |
 |
| Western blot analysis of DFF45 in HeLa (H), Jurkat (J), A431 (A), and K562 (K) whole cell lysate with DFF45 antibody at 1:1000 dilution. |
 |
| Immunocytochemistry of DFF45 in HeLa cells with DFF45 antibody at 5 µg/mL. |
 |
| Immunofluorescence of DFF45 in Hela cells with DFF45 antibody at 20 µg/mL.
Green: DFF45 Antibody (1409) Blue: DAPI staining |
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
| relevant to this product |
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| Western blot IHC ICC |
| # | SDS | Certificate | Datasheet |
|---|---|---|---|
| Please enter your product and batch number here to retrieve product datasheet, SDS, and QC information. | |||
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