Name: rabbit anti-Cyclin E1 polyclonal antibody 5435
SKU: 5435parent
Price: 376.00 USD
Availability: InStock

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	Cyclin E1
available sizes	100 µg

rabbit anti-Cyclin E1 polyclonal antibody 5435

antibody

Tested applications ELISA
Recommended dilutions Immunoblotting: use at 1:500-1:1,000.
Immunogen Peptide corresponding to amino acids 43-52 of cyclin E1.
Size and concentration 100µg and lot specific
Form liquid
Storage Instructions This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Storage buffer PBS, pH 7.4
Purity immunogen affinty purifcation
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names G1/S-specific cyclin-E1
Gene names CCNE1,CCNE1 CCNE
Protein family Cyclin family, Cyclin E subfamily
Mass 47077Da
Function FUNCTION: Essential for the control of the cell cycle at the G1/S (start) transition. {ECO:0000269\|PubMed:7739542}.
Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000269\|PubMed:7739542}.
Tissues TISSUE SPECIFICITY: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. {ECO:0000269\|PubMed:9840943}.
Structure SUBUNIT: Interacts with CDK2 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex (PubMed:15660127). Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1 (PubMed:15178429). Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation (PubMed:21952639). Interacts with INCA1 (PubMed:21540187). {ECO:0000250\|UniProtKB:Q61457, ECO:0000269\|PubMed:15178429, ECO:0000269\|PubMed:15660127, ECO:0000269\|PubMed:21540187, ECO:0000269\|PubMed:21952639}.
Post-translational modification PTM: Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. {ECO:0000269\|PubMed:14536078, ECO:0000269\|PubMed:17434132, ECO:0000269\|PubMed:21952639, ECO:0000269\|PubMed:8861947}.; PTM: Ubiquitinated by UHRF2; appears to occur independently of phosphorylation. {ECO:0000269\|PubMed:21952639}.
Target Relevance information above includes information from UniProt accession : P24864
The UniProt Consortium

Data

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Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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ELISA

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