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rabbit anti-Cyclin E1 polyclonal antibody 5435

$376.00

Antibody summary

  • Rabbit polyclonal to Cyclin E1
  • Suitable for: ELISA
  • Isotype: Whole IgG
  • 100 µg
SKU: 5435parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Cyclin E1

available sizes

100 µg

Available product – rabbit anti-Cyclin E1 polyclonal antibody 5435

antibody
Tested applications
ELISA
Recommended dilutions
Immunoblotting: use at 1:500-1:1,000.
Immunogen
Peptide corresponding to amino acids 43-52 of cyclin E1.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Storage buffer
PBS, pH 7.4
Purity
immunogen affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
G1/S-specific cyclin-E1
Gene names
CCNE1,CCNE1 CCNE
Protein family
Cyclin family, Cyclin E subfamily
Mass
47077Da
Function
FUNCTION: Essential for the control of the cell cycle at the G1/S (start) transition. {ECO:0000269|PubMed:7739542}.
Subellular location
SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:7739542}.
Tissues
TISSUE SPECIFICITY: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. {ECO:0000269|PubMed:9840943}.
Structure
SUBUNIT: Interacts with CDK2 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex (PubMed:15660127). Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1 (PubMed:15178429). Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation (PubMed:21952639). Interacts with INCA1 (PubMed:21540187). {ECO:0000250|UniProtKB:Q61457, ECO:0000269|PubMed:15178429, ECO:0000269|PubMed:15660127, ECO:0000269|PubMed:21540187, ECO:0000269|PubMed:21952639}.
Post-translational modification
PTM: Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. {ECO:0000269|PubMed:14536078, ECO:0000269|PubMed:17434132, ECO:0000269|PubMed:21952639, ECO:0000269|PubMed:8861947}.; PTM: Ubiquitinated by UHRF2; appears to occur independently of phosphorylation. {ECO:0000269|PubMed:21952639}.
Target Relevance information above includes information from UniProt accession : P24864
The UniProt Consortium

Data

No results found

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
ELISA

Documents

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