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rabbit anti-CIKS/Act1 (CT) polyclonal antibody 5494

$445.00

Antibody summary

  • Rabbit polyclonal to CIKS/Act1 (CT)
  • Suitable for: ELISA,WB,IHC-P,IF
  • Isotype: Whole IgG
  • 100 µg
SKU: 5494parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

CIKS/Act1 (CT)

available sizes

100 µg

Available product – rabbit anti-CIKS/Act1 (CT) polyclonal antibody 5494

antibody
Tested applications
WB,IHC,IHC,ICC/IF,ELISA
Recommended dilutions
Immunoblotting: use at 0.5-1 ug/mL. In immunoblots, a band of 63 kD is detected.

Positive control: Human placenta tissue lysate.
Immunogen
Peptide corresponding to aa 554-568 of human CIKS.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
E3 ubiquitin ligase TRAF3IP2 (EC 2.3.2.27) (Adapter protein CIKS) (Connection to IKK and SAPK/JNK) (E3 ubiquitin-protein ligase CIKS) (Nuclear factor NF-kappa-B activator 1) (ACT1) (TRAF3-interacting protein 2)
Gene names
TRAF3IP2,TRAF3IP2 C6orf2 C6orf4 C6orf5 C6orf6
Mass
64666Da
Function
FUNCTION: E3 ubiquitin ligase that catalyzes 'Lys-63'-linked polyubiquitination of target protein, enhancing protein-protein interaction and cell signaling (PubMed:19825828). Transfers ubiquitin from E2 ubiquitin-conjugating enzyme UBE2V1-UBE2N to substrate protein (PubMed:19825828). Essential adapter molecule in IL17A-mediated signaling (PubMed:19825828, PubMed:24120361). Upon IL17A stimulation, interacts with IL17RA and IL17RC receptor chains through SEFIR domains and catalyzes 'Lys-63'-linked polyubiquitination of TRAF6, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways (PubMed:19825828). {ECO:0000269|PubMed:19825828, ECO:0000269|PubMed:24120361, ECO:0000269|PubMed:33723527}.
Catalytic activity
CATALYTIC ACTIVITY: Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000269|PubMed:19825828};
Tissues
TISSUE SPECIFICITY: Widely expressed.
Structure
SUBUNIT: Interacts with IKBKG/NF-kappa B essential modulator, with CHUK/IKK-alpha and with IKBKB/IKK-beta (PubMed:12459498). Interacts with TRAF6; this interaction is direct (PubMed:12459498, PubMed:19825828). Interacts with IL17RA and IL17RC (PubMed:19825828, PubMed:24120361, PubMed:33723527). Interacts with IL17RB (PubMed:24120361). {ECO:0000269|PubMed:12459498, ECO:0000269|PubMed:19825828, ECO:0000269|PubMed:24120361, ECO:0000269|PubMed:33723527}.
Target Relevance information above includes information from UniProt accession : O43734
The UniProt Consortium

Data

benchmark-antibodies_anti-ciks-act1_ct_antibody_5494_1.jpg
Western blot analysis of CIKS expression in human placenta tissue lysate with CIKS antibody at 1 µg/mL.
benchmark-antibodies_anti-ciks-act1_ct_antibody_5494_2.jpg
Immunohistochemistry of CIKS in human lung tissue with CIKS antibody at 5 µg/mL.
benchmark-antibodies_anti-ciks-act1_ct_antibody_5494_3.jpg
Immunofluorescence of CIKS in Human Lung cells with CIKS antibody at 20 µg/mL.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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