| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | CIKS/Act1 (CT) |
| available sizes | 100 µg |
rabbit anti-CIKS/Act1 (CT) polyclonal antibody 5494
$445.00
Antibody summary
- Rabbit polyclonal to CIKS/Act1 (CT)
- Suitable for: ELISA,WB,IHC-P,IF
- Isotype: Whole IgG
- 100 µg
rabbit anti-CIKS/Act1 (CT) polyclonal antibody 5494
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at 0.5-1 ug/mL. In immunoblots, a band of 63 kD is detected. Positive control: Human placenta tissue lysate. |
| Immunogen Peptide corresponding to aa 554-568 of human CIKS. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names E3 ubiquitin ligase TRAF3IP2 (EC 2.3.2.27) (Adapter protein CIKS) (Connection to IKK and SAPK/JNK) (E3 ubiquitin-protein ligase CIKS) (Nuclear factor NF-kappa-B activator 1) (ACT1) (TRAF3-interacting protein 2) |
| Gene names TRAF3IP2,TRAF3IP2 C6orf2 C6orf4 C6orf5 C6orf6 |
| Mass 64666Da |
| Function FUNCTION: E3 ubiquitin ligase that catalyzes 'Lys-63'-linked polyubiquitination of target protein, enhancing protein-protein interaction and cell signaling (PubMed:19825828). Transfers ubiquitin from E2 ubiquitin-conjugating enzyme UBE2V1-UBE2N to substrate protein (PubMed:19825828). Essential adapter molecule in IL17A-mediated signaling (PubMed:19825828, PubMed:24120361). Upon IL17A stimulation, interacts with IL17RA and IL17RC receptor chains through SEFIR domains and catalyzes 'Lys-63'-linked polyubiquitination of TRAF6, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways (PubMed:19825828). {ECO:0000269|PubMed:19825828, ECO:0000269|PubMed:24120361, ECO:0000269|PubMed:33723527}. |
| Catalytic activity CATALYTIC ACTIVITY: Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000269|PubMed:19825828}; |
| Tissues TISSUE SPECIFICITY: Widely expressed. |
| Structure SUBUNIT: Interacts with IKBKG/NF-kappa B essential modulator, with CHUK/IKK-alpha and with IKBKB/IKK-beta (PubMed:12459498). Interacts with TRAF6; this interaction is direct (PubMed:12459498, PubMed:19825828). Interacts with IL17RA and IL17RC (PubMed:19825828, PubMed:24120361, PubMed:33723527). Interacts with IL17RB (PubMed:24120361). {ECO:0000269|PubMed:12459498, ECO:0000269|PubMed:19825828, ECO:0000269|PubMed:24120361, ECO:0000269|PubMed:33723527}. |
| Involvement in disease DISEASE: Psoriasis 13 (PSORS13) [MIM:614070]: A common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. {ECO:0000269|PubMed:20953186, ECO:0000269|PubMed:20953188}. Note=Disease susceptibility is associated with variants affecting the gene represented in this entry.; DISEASE: Candidiasis, familial, 8 (CANDF8) [MIM:615527]: A primary immunodeficiency disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. {ECO:0000269|PubMed:24120361}. Note=The disease is caused by variants affecting the gene represented in this entry. |
| Target Relevance information above includes information from UniProt accession: O43734 |
| The UniProt Consortium |
Data
 |
| Western blot analysis of CIKS expression in human placenta tissue lysate with CIKS antibody at 1 µg/mL. |
 |
| Immunohistochemistry of CIKS in human lung tissue with CIKS antibody at 5 µg/mL. |
 |
| Immunofluorescence of CIKS in Human Lung cells with CIKS antibody at 20 µg/mL. |
FAQ & Publications
Frequently Asked Questions
What applications has the rabbit anti-CIKS/Act1 (CT) polyclonal antibody 5494 been tested for?
This antibody has been validated for use in Western blotting (WB), immunohistochemistry (IHC-P), immunofluorescence (ICC/IF), and ELISA.
How should I store the rabbit anti-CIKS/Act1 (CT) polyclonal antibody to maintain its stability?
The antibody should be stored at -20°C and is stable for at least one year under these conditions. It is important to avoid multiple freeze-thaw cycles to preserve antibody integrity.
What is the concentration and form of the rabbit anti-CIKS/Act1 (CT) polyclonal antibody 5494 supplied in?
The antibody is supplied as a liquid at a concentration of 1 mg/mL, with a total quantity of 100 µg per vial.
Which secondary antibodies are recommended for use with this rabbit polyclonal anti-CIKS/Act1 antibody?
Compatible secondary antibodies include goat anti-rabbit IgG that are H&L chain specific and conjugated with peroxidase, biotin, or FITC, including cross-absorbed versions to reduce background.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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