| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | CIDE-B (CT) |
| available sizes | 100 µg |
rabbit anti-CIDE-B (CT) polyclonal antibody 2048
$445.00
Antibody summary
- Rabbit polyclonal to CIDE-B (CT)
- Suitable for: ELISA,WB
- Isotype: IgG
- 100 µg
rabbit anti-CIDE-B (CT) polyclonal antibody 2048
| antibody |
|---|
| Tested applications WB,ELISA |
| Recommended dilutions Immunoblotting: use at 2ug/mL. These are recommended concentrations. Enduser should determine optimal concentration for their application. Positive control: Tissue lysate of mouse liver. |
| Immunogen Peptide corresponding to aa 204- 219 of mouse CIDE-B (accession no. AF041377). |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer PBS, pH 7.4. |
| Purity peptide affinty purifcation |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| Protein names Lipid transferase CIDEB (Cell death activator CIDE-B) (Cell death-inducing DFFA-like effector B) |
| Gene names Cideb,Cideb |
| Protein family CIDE family |
| Mass 24800Da |
| Function FUNCTION: Lipid transferase specifically expressed in hepatocytes, which promotes unilocular lipid droplet formation by mediating lipid droplet fusion (PubMed:26733203). Lipid droplet fusion promotes their enlargement, restricting lipolysis and favoring lipid storage (PubMed:26733203). Localizes on the lipid droplet surface, at focal contact sites between lipid droplets, and mediates atypical lipid droplet fusion by promoting directional net neutral lipid transfer from the smaller to larger lipid droplets (By similarity). The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair (By similarity). Promotes lipid exchange and lipid droplet fusion in both small and large lipid droplet-containing hepatocytes (PubMed:26733203). In addition to its role in lipid droplet fusion, also involved in cytoplasmic vesicle biogenesis and transport (PubMed:19187774, PubMed:23297397, PubMed:30858281). Required for very-low-density lipoprotein (VLDL) lipidation and maturation (PubMed:19187774, PubMed:23297397). Probably involved in the biogenesis of VLDL transport vesicles by forming a COPII vesicle coat and facilitating the formation of endoplasmic reticulum-derived large vesicles (PubMed:23297397). Also involved in sterol-regulated export of the SCAP-SREBP complex, composed of SCAP, SREBF1/SREBP1 and SREBF2/SREBP2, by promoting loading of SCAP-SREBP into COPII vesicles (PubMed:30858281). May also activate apoptosis (PubMed:9564035). {ECO:0000250|UniProtKB:P56198, ECO:0000269|PubMed:19187774, ECO:0000269|PubMed:23297397, ECO:0000269|PubMed:26733203, ECO:0000269|PubMed:30858281, ECO:0000269|PubMed:9564035}. |
| Subellular location SUBCELLULAR LOCATION: Lipid droplet {ECO:0000269|PubMed:19187774, ECO:0000269|PubMed:26733203}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:19187774, ECO:0000269|PubMed:23297397}; Peripheral membrane protein {ECO:0000269|PubMed:19187774}; Cytoplasmic side {ECO:0000269|PubMed:19187774}. Golgi apparatus {ECO:0000269|PubMed:23297397}. Cytoplasmic vesicle, COPI-coated vesicle {ECO:0000269|PubMed:23297397, ECO:0000269|PubMed:30858281}. Note=Enriched at lipid droplet contact sites. {ECO:0000269|PubMed:26733203}. |
| Tissues TISSUE SPECIFICITY: Highly enriched in the liver. {ECO:0000269|PubMed:17646209}. |
| Structure SUBUNIT: Interacts with DFFA (By similarity). Interacts with DFFB; inhibited by DFFB (By similarity). Interacts with APOB (PubMed:19187774, PubMed:23297397). Interacts with PREB/SEC12; facilitating loading of SCAP-SREBP into COPII vesicles (PubMed:30858281). {ECO:0000250|UniProtKB:Q9UHD4, ECO:0000269|PubMed:19187774, ECO:0000269|PubMed:23297397, ECO:0000269|PubMed:30858281}. |
| Target Relevance information above includes information from UniProt accession : O70303 |
| The UniProt Consortium |
Data
 |
| Western blot analysis of CIDE-B in mouse liver tissue lysate with CIDE-B antibody at 1:500 dilution. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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| No results found | |||
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