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rabbit anti-CIDE-B (CT) polyclonal antibody 2048

$445.00

Antibody summary

  • Rabbit polyclonal to CIDE-B (CT)
  • Suitable for: ELISA,WB
  • Isotype: IgG
  • 100 µg
SKU: 2048parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

CIDE-B (CT)

available sizes

100 µg

Available product – rabbit anti-CIDE-B (CT) polyclonal antibody 2048

antibody
Tested applications
WB,ELISA
Recommended dilutions
Immunoblotting: use at 2ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentration for their application.

Positive control: Tissue lysate of mouse liver.
Immunogen
Peptide corresponding to aa 204- 219 of mouse CIDE-B (accession no. AF041377).
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Lipid transferase CIDEB (Cell death activator CIDE-B) (Cell death-inducing DFFA-like effector B)
Gene names
Cideb,Cideb
Protein family
CIDE family
Mass
24800Da
Function
FUNCTION: Lipid transferase specifically expressed in hepatocytes, which promotes unilocular lipid droplet formation by mediating lipid droplet fusion (PubMed:26733203). Lipid droplet fusion promotes their enlargement, restricting lipolysis and favoring lipid storage (PubMed:26733203). Localizes on the lipid droplet surface, at focal contact sites between lipid droplets, and mediates atypical lipid droplet fusion by promoting directional net neutral lipid transfer from the smaller to larger lipid droplets (By similarity). The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair (By similarity). Promotes lipid exchange and lipid droplet fusion in both small and large lipid droplet-containing hepatocytes (PubMed:26733203). In addition to its role in lipid droplet fusion, also involved in cytoplasmic vesicle biogenesis and transport (PubMed:19187774, PubMed:23297397, PubMed:30858281). Required for very-low-density lipoprotein (VLDL) lipidation and maturation (PubMed:19187774, PubMed:23297397). Probably involved in the biogenesis of VLDL transport vesicles by forming a COPII vesicle coat and facilitating the formation of endoplasmic reticulum-derived large vesicles (PubMed:23297397). Also involved in sterol-regulated export of the SCAP-SREBP complex, composed of SCAP, SREBF1/SREBP1 and SREBF2/SREBP2, by promoting loading of SCAP-SREBP into COPII vesicles (PubMed:30858281). May also activate apoptosis (PubMed:9564035). {ECO:0000250|UniProtKB:P56198, ECO:0000269|PubMed:19187774, ECO:0000269|PubMed:23297397, ECO:0000269|PubMed:26733203, ECO:0000269|PubMed:30858281, ECO:0000269|PubMed:9564035}.
Subellular location
SUBCELLULAR LOCATION: Lipid droplet {ECO:0000269|PubMed:19187774, ECO:0000269|PubMed:26733203}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:19187774, ECO:0000269|PubMed:23297397}; Peripheral membrane protein {ECO:0000269|PubMed:19187774}; Cytoplasmic side {ECO:0000269|PubMed:19187774}. Golgi apparatus {ECO:0000269|PubMed:23297397}. Cytoplasmic vesicle, COPI-coated vesicle {ECO:0000269|PubMed:23297397, ECO:0000269|PubMed:30858281}. Note=Enriched at lipid droplet contact sites. {ECO:0000269|PubMed:26733203}.
Tissues
TISSUE SPECIFICITY: Highly enriched in the liver. {ECO:0000269|PubMed:17646209}.
Structure
SUBUNIT: Interacts with DFFA (By similarity). Interacts with DFFB; inhibited by DFFB (By similarity). Interacts with APOB (PubMed:19187774, PubMed:23297397). Interacts with PREB/SEC12; facilitating loading of SCAP-SREBP into COPII vesicles (PubMed:30858281). {ECO:0000250|UniProtKB:Q9UHD4, ECO:0000269|PubMed:19187774, ECO:0000269|PubMed:23297397, ECO:0000269|PubMed:30858281}.
Target Relevance information above includes information from UniProt accession : O70303
The UniProt Consortium

Data

benchmark-antibodies_anti-cide-b_ct_antibody_2048_1.jpg
Western blot analysis of CIDE-B in mouse liver tissue lysate with CIDE-B antibody at 1:500 dilution.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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