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rabbit anti-Bim (IN) polyclonal antibody 1410

$445.00

Antibody summary

  • Rabbit polyclonal to Bim (IN)
  • Suitable for: ELISA,WB,IHC-P,IF
  • Isotype: IgG
  • 100 µg
SKU: 1410parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Bim (IN)

available sizes

100 µg

Available product – rabbit anti-Bim (IN) polyclonal antibody 1410

antibody
Tested applications
WB,IHC,IHC,ICC/IF,ELISA
Recommended dilutions
Immunoblotting: use at 1ug/mL.

Immunocytochemistry: use a 10-20ug/mL

These are recommended concentrations.

Enduser should determine optimal concentration for their application.

Positive control: Whole cell lysate of human K562 cells.
Immunogen
Peptide corresponding to aa 22-40 of human Bim. The sequence is identical to that of mouse and is different by one amino acid from that of rat.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4.
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein names
Bcl-2-like protein 11 (Bcl2-L-11) (Bcl2-interacting mediator of cell death)
Gene names
BCL2L11,BCL2L11 BIM
Protein family
Bcl-2 family
Mass
22171Da
Function
FUNCTION: Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis. {ECO:0000269|PubMed:11997495, ECO:0000269|PubMed:15486195, ECO:0000269|PubMed:15661735, ECO:0000269|PubMed:9430630}.
Subellular location
SUBCELLULAR LOCATION: Endomembrane system {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Note=Associated with intracytoplasmic membranes. {ECO:0000250}.; SUBCELLULAR LOCATION: [Isoform BimEL]: Mitochondrion. Note=Translocates from microtubules to mitochondria on loss of cell adherence.; SUBCELLULAR LOCATION: [Isoform BimL]: Mitochondrion.; SUBCELLULAR LOCATION: [Isoform BimS]: Mitochondrion.; SUBCELLULAR LOCATION: [Isoform Bim-alpha1]: Mitochondrion.
Tissues
TISSUE SPECIFICITY: Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are widely expressed with tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. {ECO:0000269|PubMed:12019181}.
Structure
SUBUNIT: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins, including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, BHRF1, and BCL2L2/BCLW (PubMed:11997495, PubMed:27013495, PubMed:18812174). Does not heterodimerize with proapoptotic proteins such as BAD, BOK or BAK. Identified in a complex containing BCL2L11, DYNLL1 and BCL2L1 isoform Bcl-X(L); BH3 integrity is required for BCL2L1-binding. Interacts with YWHAZ. When phosphorylated, interacts with TRIM2; this interaction is associated with ubiquitination and degradation (PubMed:21478148). Interacts with MCL1; may sequester BCL2L11 to prevent its pro-apoptotic activity (PubMed:27013495, PubMed:17389404). Interacts with GIMAP5 (PubMed:16509771). Interacts with BCL2L10/BCL-B (PubMed:23235460, PubMed:23563182, PubMed:22498477). {ECO:0000269|PubMed:11997495, ECO:0000269|PubMed:16509771, ECO:0000269|PubMed:17389404, ECO:0000269|PubMed:18812174, ECO:0000269|PubMed:21478148, ECO:0000269|PubMed:22498477, ECO:0000269|PubMed:23235460, ECO:0000269|PubMed:23563182, ECO:0000269|PubMed:27013495}.; SUBUNIT: [Isoform BimEL]: Interacts (when phosphorylated) with USP27X; the interaction leads to BCL2L11 deubiquitination and stabilization (PubMed:27013495). Interacts with humanin; the interaction prevents BIM-induced apoptosis. {ECO:0000269|PubMed:15661735}.; SUBUNIT: [Isoform BimL]: Does not interact with humanin. {ECO:0000269|PubMed:15661735}.; SUBUNIT: [Isoform BimS]: Interacts with BAX; the interaction may lead to BAX activation through conformational change (PubMed:11997495). Does not interact with humanin (PubMed:15661735). {ECO:0000269|PubMed:11997495, ECO:0000269|PubMed:15661735}.; SUBUNIT: [Isoform Bim-alpha3]: Interacts with BAX; the interaction may lead to BAX activation through conformational change. {ECO:0000269|PubMed:11997495}.
Post-translational modification
PTM: Phosphorylation at Ser-69 by MAPK1/MAPK3 leads to interaction with TRIM2 and polyubiquitination, followed by proteasomal degradation (PubMed:15486195, PubMed:21478148). Deubiquitination catalyzed by USP27X stabilizes the protein (By similarity). {ECO:0000250|UniProtKB:O54918, ECO:0000269|PubMed:15486195, ECO:0000269|PubMed:21478148}.; PTM: Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein. {ECO:0000250|UniProtKB:O54918}.
Target Relevance information above includes information from UniProt accession : O43521
The UniProt Consortium

Data

benchmark-antibodies_anti-bim_in_antibody_1410_1.gif
Western Blot Validation in Human Cell Lines
Loading: 15 µg of lysates per lane. Antibodies: BIM 1410, (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
benchmark-antibodies_anti-bim_in_antibody_1410_2.gif
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 µg of lysates per lane. Antibodies: BIM 1410, (0.5 µg/mL), BIM 3405, (5 µg/mL), beta-actin (1 µg/mL) and GAPDH (0.02 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
benchmark-antibodies_anti-bim_in_antibody_1410_3.gif
Western Blot Validation in Human Tissue
Loading: 15 µg of lysates per lane. Antibodies: BIM 1410, (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human thymusLane 2: Human colon
benchmark-antibodies_anti-bim_in_antibody_1410_4.gif
Western Blot Validation in HeLa Cells
Loading: 15 µg of lysate per lane. Antibodies: BIM 1410, (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.BIM 1410 can detect all three human isoforms, including EL, L and S isoforms.
benchmark-antibodies_anti-bim_in_antibody_1410_5.gif
Western Blot Validation in Rat Myeloma Cell line
Loading: 15 µg of rat myeloma YB2/0 cell lysate per lane. Antibodies: BIM 1410, (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
benchmark-antibodies_anti-bim_in_antibody_1410_6.gif
Immunofluorescence Validation of BIM in K562 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Bim with 1410 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
benchmark-antibodies_anti-bim_in_antibody_1410_7.jpg
Immunohistochemistry Validation of BIM in Human Skin Cancer Cells
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-BIM antibody (1410) at 20 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
benchmark-antibodies_anti-bim_in_antibody_1410_8.gif
Induced Expression Validation of BIM in Mouse Hippocampus (Tsuchiya et al., 2011)
The induction of Bim protein was detected by immunohistochemical analysis of mice after i.h. injection of epoxomicin with anti-BIM antibodies. Sections from epoxomicin-treated animals exhibited cells staining positive for Bim expression within the NeuN-positive population of neurons in the CA1 of the ipsilateral side. In contrast, Bim-positive cells were absent within the NeuNpositiveCA1 neurons on the contralateral side.
benchmark-antibodies_anti-bim_in_antibody_1410_9.gif
KD Validation of BIM in 293 Cells (Han et al., 2010)
Immunofluorescence analysis with anti-BIM antibodies was performed for BIM in 293 cells transfected with control siRNA or BIM siRNA. BIM expression was disrupted after BIM siRNA knockdown.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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